摘要
目的研究VIL-10对鼻咽癌细胞株TAP-1表达的影响。方法收集在不同时间不同浓度VIL-10处理的鼻咽癌细胞,RT-PCR检测TAP-1的表达。结果①10 ng/mlVIL-10处理的CNE-1、CNE-2和N-2-BMI-1细胞株在24、48 h时TAP-1表达无明显变化;②100 ng/ml VIL-10处理的CNE-1、CNE-2和N-2-BMI-1细胞株在24 h时TAP-1表达明显减弱。结论 100 ng/ml VIL-10在24 h可明显抑制TAP-1表达,EB病毒在鼻咽癌免疫抑制过程中起一定作用。
OBJECTIVE To investigate the influence of VIL-10 on the expression of TAP-1 in NPC cell lines.METHODS The NPC cells were treated by different concentration of VIL-10,and then harvested on different time.The expression of TAP-1 was detected by RT-PCR.RESULTS The cell lines of CNE-1,CNE-2 and N-2-BMI-1 were treated by VIL-10 with the concentration 10ng/ml,and the expression of TAP-1 in the 3 cell lines were not changed significantly at 24 hours and 48 hours.When the 3 cell lines were treated by VIL-10 with the concentration 100 ng/ml,the expression of TAP-1 at 24 hours were significantly decreased,but that at 48 hours were not significantly decreased.CONCLUTION The expression of TAP-1 were inhibited by VIL-10 with the concentration 100 ng/ml at 24 hours in NPC cell lines.The results indicate that EB Virus could induce immunosuppression in NPC patients.
出处
《中国耳鼻咽喉头颈外科》
2012年第5期255-257,共3页
Chinese Archives of Otolaryngology-Head and Neck Surgery
基金
云南省社会发展科技计划(应用基础研究项目)(2009ZC119M)
2011年云南省卫生科技内设研究机构项目资助(2011WS0068)
云南省社会发展科技计划(应用基础研究重点项目)(2009CC026)联合资助
关键词
鼻咽肿瘤
载体蛋白质类
逆转录聚合酶链反应
疱疹病毒4型
人
病毒白介素-10
Nasopharyngeal Neoplasms
Carrier Proteins
Reverse Transcriptase Polymerase Chain Reaction
Herpesvirus 4
Human
virus interleukin-10
