EGCG预防苯并芘诱发大鼠肺癌作用的研究

Prevention effect of EGCG in rat's lung cancer induced by benzopyrene

目的:研究EGCG对苯并芘诱发大鼠肺癌的预防作用。初步探讨其预防作用是否与NF-κB和Ki-67在肺癌中的表达有关。方法:实验分3组,EGCG干预组(简称干预组)、非EGCG干预组(简称非干预组)和对照组,前两组采用胸壁穿刺肺内注射苯并芘的方法制造SD大鼠肺癌模型,其中干预组加入EGCG干预因素,观察各组大鼠肺癌的成瘤率,免疫组化检测大鼠肺癌组织NF-κB p50、Ki-67的表达情况。结果:干预组和非干预组两组间成瘤率〔分别为35.0%(7/20)和80.0%(16/20)〕差异有统计学意义,P<0.01;肺癌组织与非肺癌组织NF-κB p50(χ2=25.377)、Ki-67(χ2=19.157 7)的表达差异有统计学意义,P<0.01;干预组、非干预组和对照组3组中NF-κB p50高表达率分别为35.0%、90.0%和0,Ki-67高表达率分别为40.0%、85.0%和0。干预组和非干预组两组比较,NF-κB p50高表达率差异有统计学意义,χ2=12.906 7,P<0.01,非干预组和对照组两组比较,NF-κB p50高表达率差异有统计学意义(χ2=32.727 3,P<0.01);干预组和非干预组两组比较,Ki-67高表达率差异有统计学意义(χ2=8.64,P<0.01),非干预组和对照组两组比较,Ki-67高表达率差异有统计学意义(χ2=29.565 2,P<0.01);EGCG干预组中有或无继发肿瘤时,NF-κB p50与Ki-67表达差异有统计学意义(分别为χ2=12.175,P<0.01;χ2=9.377,P<0.01);无EGCG干预组有或无继发肿瘤者,NF-κB p50与Ki-67表达也差异有统计学意义(分别为χ2=8.889,P<0.01;χ2=4.804,P<0.05)。结论:EGCG对苯并芘诱发大鼠肺癌有明显的预防作用,其作用机制可能与其抑制NF-κB p50、Ki-67的表达有关。

OBJECTIVE：To Study EGCG＇s prevention effect in rats lung cancer induced by benzopyrene,and preliminary investigate the association of prevention effect with the expression of NF-κB and Ki-67 in rats lung cancer. METHODS： The experiment was divided into three groups： Intervention group, non-intervention group ang control group. Intervention group and non-intervention group rats received percutaneous lung puncture injection of benzopyrene to create SD rats lung cancer model. Control group and intervention group is with EGCG intervention. The tumor formation rate among each group was observed. The expression of nuclear facto-κB and Ki-67 were examined by SP immunohistochemistry in all the rats lung cancer tissue. RESULTS： The percentage of tumor formation between intervention group and non-intervention group [respectively 35.0% （7/20） and 80.0%（16/20）] was statistically significant （P〈0.01）. The expression of NF-κB p50 （Х^2=25. 377） and Ki-67 （Х^2= 19. 157 7）in lung cancer tissue and non-cancer tissue were statistically significant （P〈0.01）. The high expression rate of NF-κB p50 in intervention group, non-intervention group and control group was respectively 35.0 %, 90.0 %, 0 and the high expression rate of Ki-67 in intervention group, non-intervention group and control group was respectively 40. 0%, 85.0% and 0. The high expression rate of NF-κB p50 between intervention group and non-intervention group was statistically significant （Х^2 = 12. 906 7,P〈0.01）. The high expression rate of NF-κB p50 between non-intervention group and control group was statistically significant （Х^2 = 32. 727 3,P〈0.01）. The high expression rate of Ki-67 between intervention group and non-intervention group was statistically significant （Х^2 = 8.64, P〈0. 01）. The high expression rate of Ki-67 between non-intervention group and control group was statistically significant （Х^2 = 29. 565 2, P〈0.01）. In＇ EGCG intervention group with or without secondary tumor,the expression rate of both NF-κB p50 and Ki-67 were statistically significant （respectively Х^2= 12. 175, P〈0.01.Х^2 = 9. 377, P〈 0. 01） ;In non-EGCG intervention groups with or without secondary tumor, the expression rate of both NF-κB p50 and Ki-67 were statistically significant （ respectively Х^2= 8.8 89, P〈 0.01 ; Х^2= 4.804, P 〈 0.0 5 ）. CONCLUSIONS .. EGCGhas preventioneffect in rats lung cancer induced by benzopyrene. It may associate with the inhibition of NF-κB p50 and Ki-67＇s expression.