钙离子结合蛋白S100A9在乳腺癌中表达及临床意义

Expression and clinical significance of calcium-binding protein S100A9 in breast cancer

目的:探讨钙离子结合蛋白S100A9在乳腺癌患者中的表达及临床意义。方法:选取39例乳腺癌患者术前、15例术后血清及10例健康女性血清,12例手术切除肿瘤组织标本,应用ELISA检测乳腺癌患者血清S100A9水平,采用免疫组织化学方法检测乳腺癌组织中S100A9的表达,分析S100A9表达与临床病理特征的关系。结果:S100A9在乳腺癌组患者血清中表达水平高于健康组(P<0.01),有淋巴结转移乳腺癌组血清S100A9表达水平高于无淋巴结组(P<0.01),乳腺癌患者术后S100A9血清表达水平降低(P<0.01)。乳腺癌患者血清中S100A9水平与患者年龄、肿瘤最大径、组织学分级和病理类型有关(P<0.01)。S100A9在乳腺癌组织中表达明显高于癌旁正常组织。结论:S100A9表达水平在乳腺癌血清和组织中明显升高,表明S100A9可作为乳腺癌患者诊断和疗效观察指标。

AIM： To investigate the expression and clinical significance of calcium-binding protein S100A9 in the patients of breast cancer. METHODS： The serum S100A9 level from 39 cases of preoperative breast cancer, 15 post- operative patients, and l0 healthy women was detected by ELISA. Immunohistochemistry was used to detect the SI00A9 expression in 12 specimens of breast cancer tissues. RESULTS： The serum SI00A9 level was signifi- cantly higher in the preoperative breast cancer patients than that in the healthy women （P 〈 0.01 ） and the postoperative breast cancer patients（ P 〈 0.01 ）. The serum S100A9 level of the breast cancer patients with lymph node metastasis was significantly higher than those without lymph node me- tastasis （P 〈 0.01 ）. S100A9 level in the serum was related to patient age, tumour size, histological grade, and patho- logical type （ P 〈 0.01 ）. The expression of S100A9 protein in breast cancer tissues was significantly higher than that in paracancerous benign breast tissues. CONCLUSION： The expression of S100A9 significantly increases in the serum and tissues of patients with breast cancer, which indicates that S100A9 may function as a marker of diagnosis and treatment effect in breast cancer.