雷奈酸锶在模拟微重力环境对成骨细胞凋亡的影响

The effects of strontium ranelate on osteoblast apoptosis in simulated microgravity

观察雷奈酸锶这种新型的抗骨质疏松药,在模拟微重力环境对成骨细胞凋亡的影响。方法细胞凋亡是利用4,6-二氨基-2-苯基吲哚所染细胞核形态的改变来辨认,利用旋转细胞培养系统模拟微重力环境。结果模拟微重力环境可以促进成骨细胞的凋亡,雷奈酸锶可以纠正模拟微重力环境对成骨细胞凋亡的影响。结论在模拟微重力环境雷奈酸锶对成骨细胞生存具有保护作用,这为雷奈酸锶治疗微重力环境骨丢失提供了理论和实验证据。

Objective To investigate the role of strontium ranelate, the novel antiosteoporotic agent, in regulating the apoptosis of osteoblasts under simulated microgravity. Methods Apoptotic cells were identified on the basis of nuclear morphology with 4,6-diamidino-2 phenylindole （DAPI） staining and rotating cell culture system was used to simulate microgravitational effect. Results Simulated microgravity increased apoptosis of osteoblasts cells and this could be reversed by strontium ranelate treatment. Conclusion Here we demonstrate that strontium ranelate plays a protective role in osteoblast survival in simulated microgravity environment. This provides a theoretical and experimental evidence for the treatment of microgravity-induced bone loss with strontium ranelate.