蛹虫草液体发酵与胞内多糖提取研究

Study on the Liquid-submerged Fermentation and Polysaccharide Extraction of Cordyceps Militaris Ycelium

为优化蛹虫草的液体发酵及胞内多糖提取工艺,采用单因素对比试验法,确定蛹虫草液体发酵的最佳碳源为3%的玉米粉、最佳氮源为2%的蛋白胨。分别采用常规热水浸提、微波辅助水浸提、超声波水提法对蛹虫草菌丝体胞内多糖提取工艺进行研究,确定超声波水提法的提取效果最好,多糖得率为4.03%。

Through carbon, nitrogen single-factor comparing experiment, cornmeal was determined as the best carbon, the optimal addition was 3%; peptone as the best nitrogen source, the optimal addition was 2%. Cordyceps polysaccharide was extracted with three methods： boiling water bath, microwave and ultrasonic method. Comparisons showed that among the three methods, ultrasonic was the best one, the extraction rate of polysaccharide could reach 4.03%. The study provided a theoretical basis for liquid-submerged fermen- tation and polysaccharide extraction of Cordyeeps militaris mycelium.