摘要
采用Ｗａｔｅｒｓ高效液相色谱系统，硅胶色谱柱（３．９ｍｍ× ３００ｍｍ）；流动相为异丙醇／正己烷（４／３，ｖ／ｖ）和异丙醇／正己烷／水（８／６／１．５，ｖ／ｖ／ｖ），梯度洗脱。用这种方法能够将磷脂胶囊的磷脂各组分完全分离，根据保留时间可定性确定组分；根据峰面积可以测定各组分的相对含量；根据标准样品中磷脂组分的含量可以测定样品中主要磷脂的绝对含量。
This paper dealt with qualitative and quantiative analysis of the compositions of the soybean phospholipids capsule by high performance liquid chromatography (HPLC) .We adopted Waters HPLC system using a silica column (3.9mm×300mm) .Gradient elution was carried out with isopropanol hexance (4/3 1 v/v) and isopropanal-hexance-water (8/ 6/1. 5, v/v/v) By this method the compositions of soybean phospholipids in soybean phospholipids capsule could be separated completely and the composition defined qualitatively according to the retention time. The relative content of each composition could be determined according to the peak area, and the absolute content of the predominant compositions also determined according to the content of each phospholipid in the standard sample.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2000年第5期53-54,共2页
Food Science
基金
浙江省自然科学基金!(批准号398025)
