期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

microRNAs在肾透明细胞癌组织中的表达及意义 被引量：4

Expression and significance of miRNAs in renal clear cell carcinoma tissues
下载PDF
导出
摘要 目的探讨microRNA(miRNA)在肾透明细胞癌组织中的表达及其临床意义。方法用实时荧光定量PCR技术检测20例肾透明细胞癌患者癌组织与癌旁组织中miRNA-20b、-21、-34a、-210、-141、-200c、200b、-514、-532-5p的表达变化。结果与癌旁组织相比,肾透明细胞癌患者癌组织中miRNA-21、-34a、-210呈高表达(P<0.05),miRNA-141、-200c呈低表达(P<0.05),miRNA-20b、-200b、-514、-532-5p则无差异。miRNA-34a随着肾癌病理分级的增高而增高。结论miRNA-21、-34a、-210、mir-141、-200c在肾透明细胞癌患者癌组织中表达存在差异,可能在肾透明细胞癌中起重要作用。 Objective To study miRNAs expression in renal clear cell carcinoma and its clinical significance.Methods The expression levels of miRNA-20b,miRNA-21,miRNA-34a,miRNA-210,miRNA-141,miRNA-200c,miRNA-200b,miRNA-514 and miRNA-532-5p in renal clear cell carcinoma tissues and their corresponding adjacent tissues were analyzed with qRT-PCR;their relationships with clinical pathological features were analyzed with statistical method.Results Compared with adjacent tissue,miRNA-21,miRNA-34a,and miRNA-210 were high-expressed in renal cell carcinoma while miRNA-141 and miRNA-200c were low-expressed（P0.05）,the expression levels of miRNA-20b,miRNA-200b,miRNA-514 and miRNA-532-5p kept unchanged.The expression level of miRNA-34a increased with the tumor grade.Conclusion miRNA-21,miRNA-34a,miRNA-210,miRNA-141 and miRNA-200c were differentially expressed in renal clear cell carcinoma;they may be used as potential diagnostic and therapeutic targets for renal cell carcinoma. To study miRNAs expression in renal clear cell carcinoma and its clinical significance.Methods The expression levels of miRNA-20b,miRNA-21,miRNA-34a,miRNA-210,miRNA-141,miRNA-200c,miRNA-200b,miRNA-514 and miRNA-532-5p in renal clear cell carcinoma tissues and their corresponding adjacent tissues were analyzed with qRT-PCR;their relationships with clinical pathological features were analyzed with statistical method.Results Compared with adjacent tissue,miRNA-21,miRNA-34a,and miRNA-210 were high-expressed in renal cell carcinoma while miRNA-141 and miRNA-200c were low-expressed（P0.05）,the expression levels of miRNA-20b,miRNA-200b,miRNA-514 and miRNA-532-5p kept unchanged.The expression level of miRNA-34a increased with the tumor grade.Conclusion miRNA-21,miRNA-34a,miRNA-210,miRNA-141 and miRNA-200c were differentially expressed in renal clear cell carcinoma;they may be used as potential diagnostic and therapeutic targets for renal cell carcinoma.
作者 杨阳 张运津 崔苏萍 娄远蕾 谢安 汪泱
机构地区 南昌大学第一附属医院泌外研究所 江西广济医院 南昌大学第一附属医院泌尿外科
出处 《实验与检验医学》 CAS 2012年第2期119-122,共4页 Experimental and Laboratory Medicine
基金 国家自然科学基金(编号:30960385) 江西省卫生厅基金资助项目(编号:20123025)
关键词 肾透明细胞癌 MIRNA 实时荧光定量PCR Renal clear cell carcinoma miRNAs Quantitative real-time PCR
分类号 Q522 [生物学—生物化学] R737.11 [医药卫生—肿瘤]
  • 相关文献

参考文献19

  • 1Chow TF, Youssef YM, Lianidou E, et al. Differential expression profiling of micreRNAs and their potential involvement in renal cell carcinoma pathogenesis[J]. Clin Biochem, 2010, 43(1-2): 150- 158.
  • 2Huang Y, Dai Y, Yang J, et al.Mieroarray analysis of mieroRNA ex- pression in renal clear cell carcinoma [J]. Eur J Surg Oneol,2009, 35(10): 1119-1123.
  • 3Jurtg M, Mollenkopf ILl, Grimm C,et al. MicroRNA profihng of clear cell renal cell cancer identifies a robust signature to define renal malignancy[J]. J Cell Mol Med,2009, 13( 9B):3918-3928.
  • 4Juan D, Alexe G, Antes T, et al. Identification of a microRNA pan- el for clear-cell kidney cancer[J]. Urology,2010,75(4) : 835-841.
  • 5Yi Z, Fu Y, Zhao S,et al. Differential expression of miRNA pat- terns in renal cell carcinoma and nontumorous tissues[J]. J Cancer Res Clin Oncol,2010, 136(6) : 855-862.
  • 6Volinia S, Calin GA, Liu CG,et al. A microRNA expression signa- ture of human solid tumors defines cancer gene targets [J]. Proc Natl Acad Sci U S A,2006, 103(7):2257-2261.
  • 7Zhu S, Si ML, Wu H,et al. MicroRNA-21 targets the tumor sup- pressor gene tropomyosin 1 (TPM1) [J]. J Biol Chem,2007, 282 (19): 14328-14336.
  • 8Meng F, Henson R, Wehbe-Janek H,et al. MieroRNA-21 regu- lates expression of the PTEN tumor suppressor gane in human hepatoeellular cancer[J]. Gastreenterology,2007, 133(2) : 647-658.
  • 9刘芬,吴珏,娄远蕾,阮琼芳,李勇,崔苏萍,汪泱.肾缺血对缺氧诱导microRNAs及VEGF-NOTCH信号分子的影响[J].中华急诊医学杂志,2011,20(4):380-384. 被引量：5
  • 10高法梁,娄远蕾,阮琼芳,涂伟,吕世刚,潘长福,吴雷,汪泱,邓志锋.缺血性脑损伤大鼠MicroRNA 210的动态变化[J].实验与检验医学,2010,28(3):230-232. 被引量：9

二级参考文献40

  • 1马贵喜,李鸣皋,韩磊,刘玉,李靖,蒙果,黄友章.缺氧对血管内皮细胞内Ca^(2+)的影响以及合贝爽的保护作用[J].山东医药,2007,47(16):18-19. 被引量：6
  • 2Ivan M,Harris AL,Martelli F,et,al.Hypoxia response and microRNAs:no longer two separate worlds[J].J Cell Mol Med 2008,12(5):1426-1431.
  • 3Com PG.Hypoxic regulation of miR-210:shrinking targets expand HIF-1' s influence[J].Cancer Biol Ther 2008,7 (2):265-267.
  • 4Fasanaro P,D' Alessandra Y,Di Stefano V,et,al.MicroRNA-210modulates endothelial cell response to hypoxia and inhibits the receptor tyrosine kinase ligand Ephrin-A3[J].J Biol Chem 2008,283(23):15878-15883.
  • 5Beck H,Plate KH.Angiogenesis after cerebral ischemia[J].Acta Neuropathol,2009,117 (5):481-496.
  • 6Wienholds E,Plasterk RH.MicroRNA function in animal development[J].FEBS Lett,2005,579(26):5911-5922.
  • 7van Solingen C,Seghers L,Bijkerk R,et,al.Antagomir-Mediated Silencing of Endothelial Cell Specific MicroRNA-126 Impairs Ischemia-Induced Angiogenesis[J].J Cell Mol Med,2009,13(8):1577-1585.
  • 8Shen J,Yang X,Xie B,et,al.MicroRNAs regulate ocular neovascularization[J].Mol Ther,2008,16(7):1208-1216.
  • 9Bonauer A,Carmona G,Iwasaki M,et,al.MicroRNA-92a controls angiogenesis and functional recovery of ischemic tissues in mice[J].Science,2009,324(5935):1710-1713.
  • 10Jeyaseelan K,Lim KY,Armugam A.MicroRNA expression in the blood and brain of rats subjected to transient focal ischemia by middle cerebral artery occlusion[J].Stroke 2008,39(3):959-966.

共引文献16

同被引文献32

  • 1刘中娟,林嘉友.单核细胞趋化蛋白-1表达与狼疮肾炎[J].中国医学科学院学报,2005,27(4):491-495. 被引量：2
  • 2Filip A.miRNA--new mechanisms of gene expression control [J]. Postepy Biochem,2007,53 (4):413-419.
  • 3Kato M, Zhang J, Wang M,et al.MicroRNA-192 in diabetic kidney glomeruli and its function in TGF-β-induced collagen expression via inhibition of E-box repressors [J].Proc Nail Acad Sci U S A, 2007,104(9):3432 - 3437.
  • 4Kato M, Wang L, Putta S,et al.Post-transcriptional up-regulation of Tsc-22 by Ybxl, a target of miR-216a, mediates TGF-{beta}- induced collagen expression in kidney ceils [J]. J Bid Chem,2010, 285(44):34004-34015.
  • 5Long JY, Wang Y, Wang WJ,et al. MicroRNA-29c is a signature microRNA under high glucose conditions which targets sprouty ho- molog 1, and Its In vivo knockdown prevents progression of dia- betic nephropathy[J]. J Bid Chem, 2011,286(13):11837-11848.
  • 6Long JY, Wang Y, Wang WJ,et al.Identification of microRNA-93 as a novel regulator of vascular endothelial growth factor in hyper- glycemic conditions[J].Biol Chem, 2010,285(30):23457-23465.
  • 7Filip A.miRNA-new mechanisms of gene expression control [J]. Postepy Bioehem,2007,53(4) :413-419.
  • 8Vosa U,Vooder T,Kolde R,et al. Identification of miR-374a as a prognostic marker for survival in patients with early-stage nons- mall cell lung cancer [J]. Genes Chromosomes Cancer,2011,50 (10):812-22.
  • 9Li JY,Zhang Y,Zhang WH,et al. Effects of differential distribu- tion of microvessel density,possibly regulated by miR-574a,on breast cancer prognosis [J]. Asian Pac J Cancer Prev,2013,14(3): 1715-1720.
  • 10Cai J,Guan H,Fang L,et al. MicroRNA-374a activates Wnt/13- catenin signaling to promote breast cancer metastasis[J]. J Clin In- vest, 2015,123(2) : 566-779.

引证文献4

二级引证文献22

实验与检验医学
2012年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部