通心络对溶血磷脂酰胆碱所致人脐静脉内皮细胞损伤的影响

The effects of Tong Xin-Luo on human umbilical vein endothelial cells impaired by lysophosphatidylcholine

目的观察通心络（TXL）对溶血磷脂酰胆碱（LPC）致人脐静脉内皮细胞损伤作用的影响。方法制备通心络含药血清，人脐静脉内皮细胞培养，分为（1）正常对照组；（2）LPC损伤组；（3）通心络血清组；（4）通心络＋LPC干预组。观察细胞形态变化，MTr法检测各组细胞OD值。结果与对照组（0．380±0．023）相比，溶血磷脂酰胆碱（0．320±0．024）明显降低细胞活性，促进细胞死亡（P〈0．05）；通心络干预后（0．424±0．034），细胞活性明显增加，细胞死亡减少（P〈0．05）。结论通心络对溶血磷脂酰胆碱致人脐静脉内皮细胞损伤具有保护作用。

Objective To investigate the effects of Tong Xin-Luo on cultured human umbilical vein endothelial cells （HUVECs） impaired by Lysophosphatidylcholine. Methods The herbage-contained serum of TXL was prepared, HUVECs were cultured in vitro. The study was designated to 4 group ： normal control, LPC group, TXL group, and TXL ＋ LPC intervened group. The cell function was determined by cell morphology and MTF colorimetric assay. Results Compaired with normal control group （ 0. 380 ± 0. 023 ）, LPC （0. 320 ± 0. 024 ） could significantly decrease the cells activity, promote cells death （ P 〈 0.05 ）. After TXL intervened（0. 424 ± 0. 034） , cells activity was significantly increased, cells death was d significantly decreased（ P 〈0. 05 ）. Conclusions Tong Xin-Luo could protect human umbilical vein endo- thelial cells function by against the LPC-induced damage.