17-β雌二醇对受力后盆底功能障碍性疾病患者阴道壁成纤维细胞的生物学影响

Study on effect of 17-β estradiol on cultured vaginal wall flbroblasts from patient with pelvic floor dysfunction after stretch

目的研究雌激素对体外培养的盆底功能障碍性疾病（pelvicfloordysfunction，PFD）患者阴道壁成纤维细胞受力后胶原蛋白I（ColI）、类赖氨酰氧化酶1（LOXLl）和纤维粘连蛋白（FN）表达的影响，探讨其在PFD发病中的作用。方法选取2009年10月至2010年9月河北医科大学第二医院接受手术治疗的女性PFD患者的阴道壁组织12例。采用组织块法和酶消化法进行阴道壁成纤维细胞的原代培养，传代后进行细胞受力，加药，提取细胞mRNA，测定ColI、LOXLl及FN的表达水平。结果受力前、后ColI（1．1872±0．0733VS1．5035±0．0733，t=-4．815，P〈0．01）、LOXLl（0．7724±0．1873VS1．0855-I-0．0805，t=-5．111，P〈0．01）表达上升，其2，!异有统计学意义。FN（0．4290±0．1168VS0．4215±0．0830）的表达比较差异无统计学意义（P〉0．05）。高浓度的17-β雌二醇作用下，ColI（3．0809±0．1862）、LOXL1（1．5863±0．3241）、FN（1．1418±1．0030）的表达均上升，与对照组比较差异均有统计学意义（P〈0．01）。结论17-B雌二醇使受力后的PFD患者阴道壁成纤维细胞合成细胞外基质（Extracellularmatrix，ECM）成分增加。力和雌激素在PFD的发病机制中可能起了比较重要的作用。

Objective To study the effect of 17-βestradiol on Collagen I ,lysyl oxidase like 1 （LOXL1）, fibrinectin（FN） after stretch in the pelvic function dysfunction （PFD）patients vaginal wall fibroblasts cultured in vitro, and the effect in PFD pathogenesis. Methods Twelve patients with pelvic function dysfunction who under surgery in the second Hospital, Hebei Medical University from October 2009 to September 2010 were collected vaginal wall organization in this study. Use of tissue and collagenase digestion method for primary culture in the vaginal wall fibroblasts. After stretch with different concentrations of 17-β estradiol cultured fibroblasts. Total mRNA was extracted from fibroblasts and gene expression of Col I , LOXL1, FN were measured. Results After stretch , the expression of Col I （ 1. 1872 ±0. 0733 vs 1. 5035 ±0.0733, t = -4.815, P 〈0. 01）,LOXLl（0.7724 ±0. 1873 vs 1.0855 ±0.0805, t = -5. 111, P 〈 0. 01 ） were significant higher than before（ P 〈0. O1 ） , the expression of FN （0. 4290 ±0. 1168 vs 0. 4215 ±0. 0830）was not significantly different （ P 〉 0. 05 ）. The expression of all factor Col I （3. 0809 ± 0. 1862） ,LOXL1 （1. 5863 ±0. 3241 ） ,FN（ 1. 1418 ± 1. 0030） in high drug concentrations of 17-β estradiol were increased significant compared with controlled groups （ P 〈 0. 01 ）. Conclusions 17-β estradiol in- creased synthesis of extracellular matrix components from cultured vaginal wall fibroblasts of patient with pel vic floor dysfunction after stretch. Stretch and estrogen may play an important role in PFD pathogenesis.