内脂素在2型糖尿病伴高甘油三酯血症患者网膜脂肪组织中的表达及与血脂的关系

Study on expression of visfatin mRNA in abdominal omental adipose tissue and its relationship with blood lipid in type 2 diabetics

目的探讨内脂素（visfatin）基因在2型糖尿病（T2DM）患者网膜脂肪组织中的表达情况及与血脂的关系。方法选取2型糖尿病患者161例，分为高甘油三酯及正常甘油三酯两组。采用Northernblot印迹技术研究两组的网膜脂肪组织中visfatinmRNA的表达水平，并测定血清visfa-tin浓度、空腹血糖、血脂谱及其他生化指标。结果（1）2型糖尿病伴高甘油三酯血症组血清visfatin增高[（129．074-21．35）ng／mLVS（101．654-15．23）ng／mL，t=2．295，P〈0．05]，网膜组织的visfatinmRNA表达量高于正常甘油三酯组（P〈0．05）。（2）血清visfatin与甘油三酯（B=0．592，P〈0．05）及空腹血糖（B=0．763，P〈0．01）呈正相关，网膜脂肪组织visfatin与空腹血糖、甘油三酯等均无相关。结论T2DM患者血清visfatin可能与甘油三酯代谢相关。

Objective To investigate the expression of visfatin mRNA in abdominal omental adi- pose tissue and its relationship with blood lipid in type 2 diabetes mellitus. Methods 161 type 2 diabetic patients were divided into two groups according to triglyceride （ TG）, high triglyceride group （ TG I〉 1.7 mmol/L） and normal triglyceride group（ TG 〈 1.7 mmol/L）. The expression of visfatin mRNA in abdomi- nal omental adipose tissue was measured with Northern Blot. Visfatin plasma concentration, fasting plasma glucose （FPG）, blood lipid profiles and other biochemical indicators were also measured. Results Com- paring with normal triglyceride group, the diabetic patients in high triglyceride group had significantly in- creased levels of visfatin plasma concentration[ （ 129. 07 ± 21.35 ） ng/mL vs （ 101.65± 15. 23 ） ng/mL, t = 2. 295, P 〈 0. 05 ] and mRNA expression in omental adipose tissue（ P 〈 0. 05 ）. Visfatin plasma concentration was positively correlated with TG（ β= 0. 592, P 〈 0. 05 ） and FPG（ β= 0. 763, P 〈 0. 01 ）. Visfatin mRNA expression in omental adipose tissue had no correlation with FPG, TG and other biochemical indicators. Conclusions The plasma visfatin level in rF2DM patients may be associated with triglyceride metabolism.