莱菔硫烷对人小细胞肺癌NCI—H446细胞增殖、侵袭以及MMP-9活性的影响

Effect of sulforaphane on human small cell lung cancer NCI-H446 cells proliferation, invasion and the activity of MMP-9

目的探讨莱菔硫烷（sulforaphane，SFN）对人小细胞肺癌NCI—H446细胞增殖、侵袭以及基质金属蛋白酶-9（matrixmetalloproteinase-9，MMP-9）活性的影响。方法体外培养NCI—H446细胞，用0、25、50、100txmol／LSFN处理24～72h后，MTT法检测细胞的增殖情况；小室侵袭实验分析细胞的侵袭力改变，明胶酶谱实验检测MMP-9的酶活性变化。结果经25、50、100Ixmol／LSFN处理24～72h后，NCI．H446生长均能受到不同程度抑制。其中72h后，25、50、100μmol／LSFN组NCI．H446细胞的抑制率分别为（11．1±2．26）％，（25．2±3．24）％和（44．6±4．2）％，与溶剂对照组相比差异具有统计学意义（t=10．685，8．417，5．264，P〈0．05）。25、50、100μmol／LSFN作用NCI—H446细胞24h后，NCI—H446细胞穿膜数分别为（48．6±1．84）％，（35．4±2．22）％和（27．8±1．36）％，与溶剂对照组[（68．4±2．21）％]相比差异具有统计学意义（t=6．341，5．562，4．925，P〈0．05）。明胶酶谱实验显示，25、50、100ixmol／LSFN处理后，MMP-9活性的灰度值分别为764±18．4，685±14．74和638±21．54，与对照组相比（822±12．53），差异有统计学意义（t=4．971，7．582，11．235，P〈0．05）。结论SFN对肺癌NCI—H446细胞生长、侵袭力及MMP-9的活性具有抑制作用。

Objective To investigate the effects of sulforaphane （SFN） on proliferation and invasion of human small cell lung cancer NCI-H446 and the activity of matrix metalloproteinase （MMP） -9. Methods NCI-H446 cells were cultured with 0, 25, 50,100 μmol/L SFN for 24 - 72 h, then MTT assay was employed to detect cell proliferation. Chamber invasion assay was used to study the cell invasion, and gelatin zymography assay was implied in MMP-9 enzyme activity. Results After treatment of 25, 50, 100 μmol/L SFN, the growth of NCI-H446 cells were inhibited. When cells were incubated with 25, 50, 100μmol/L of SFN for 72h, the inhibition ratio was （11.1 ±2.26）%, （25.2 ±3.24）% and （44.6 ± 4. 2） %, respectively, the difference was statistically significant compared with the solvent control group （ t = 10. 685,8. 417,5. 264, P 〈 0.05 ）. Chamber invasion assay showed that NCI-H446 cell invasion could be reduced. 25, 50, 100 μmol/L of SFN could decrease the trans-membrane cells to （48. 6 ± 1.84） %, （ 35.4 ± 2. 22） % and （27.8 ± 1.36） %, and it was statistically significant compared with the solvent control group （ t = 6. 341,5. 562,4. 925, P 〈 0. 05）, respectively. In addition, MMP-9 activity was significantly inhibited by SFN. 25, 50, 100 μmol/L of SFN could decrease the gray value of MMP-9 to 764 ± 18.4,685 ± 14. 74 and 638 ± 21.54 （control group 822 ± 12. 53, t = 4. 971,7. 582, 11. 235, respectively,SFN can inhibit NCI-H446 cells growth, invasion and the activity of MMP-9