摘要
对玉米醇溶蛋白湿法糖基化的改性工艺进行了初步研究,分析了不同糖基供体、温度、底物浓度对接枝度、可溶性蛋白和游离氨基含量的影响。结果表明,葡萄糖作为糖基供体,在90℃、蛋白与葡萄糖质量比为1:2时进行糖基化反应,接枝度可达到38.34%,可溶性蛋白含量为1.22mg/mL,可溶性蛋白含量提高了1.84倍。
Modification process of glycosylation of zein by water-heating was preliminarily studied.Effects of different glycosyl donor,temperature,concentration of substrate on the conjugation degrees and the solubility and free amino of the conjugates were investigated.Results showed that under these conditions（temperature 90℃,weight ratio of protein to glucose 1：2） with glucose as glycosyl donor,conjugation degrees achieved 38.34% and content of soluble protein was 1.22mg/mL which rised 1.84 times than original zein.
出处
《中国食物与营养》
2012年第4期55-58,共4页
Food and Nutrition in China
基金
国家高新技术研究发展计划"玉米绿色供应链技术创新与装备研制"(项目编号:2008AA100802)
