雌激素剂量依赖性促进小鼠骨髓间充质干细胞的成骨分化

Estrogen promotes osteogenic differentiation of mouse bone marrow mesenchymal stem cells in a dose-dependent manner

背景:绝经后骨质疏松的发病与雌激素水平的下降关系密切。目的:观察不同浓度雌激素对小鼠骨髓间充质干细胞成骨分化能力的影响,及其与微小RNA-26a的关系。方法:取小鼠股骨与胫骨骨髓,全骨髓贴壁法获得并纯化骨髓间充质干细胞,分别以0,10-10,10-9,10-8,10-7,10-6 mol/L的雌二醇对其成骨诱导过程进行干预。结果与结论:雌二醇对骨髓间充质干细胞的增殖能力影响不明显,但可明显提高其成骨能力;同时雌二醇可促进骨髓间充质干细胞成骨基因RUNX2,OCN mRNA及RUNX2,SP7蛋白的表达,以10-9 mol/L雌二醇的作用最明显,但10-9mol/L雌二醇促进微小RNA-26a mRNA表达的能力最弱。说明雌二醇可剂量依赖性促进骨髓间充质干细胞的成骨分化,微小RNA-26a可能在此过程中发挥作用。

BACKGROUND： The pathogenesis of postmenopausal osteoporosis and estrogen deficiency is closely related. OBJECTIVE： To investigate the effects of different concentrations of estrogen on osteogenic differentiation of mouse bone marrow mesenchymal stem cells （BMSCs） and the relationship with miRNA-26a. METHODS： Mouse femoral and tibial bone marrow was taken and BMSCs were isolated and purified by the whole bone marrow adherence method. Estrogen at different concentrations （0, 10-10, 10-9, 10-8, 10-7, 10-6 mol/L） was used for osteogenic induction. RESULTS AND CONCLUSION： Estrogen did not greatly influence the proliferation of BMSCs, but it could significantly enhance the osteogenic differentiation capability of BMSCs. At the same time, estrogen, in particular at a concentration of 10-9 mol/L, could promote RUNX2, OCN mRNA and RUNCX2, SP7 protein expression in BMSCs. However, 10-9 mol/L estrogen promoted miRNA-26a mRNA expression at the weakest capability. These findings suggest that estrogen can promote osteogenic differentiation of BMSCs in a dose-dependent manner, and microRNA-26a possibly exerts effects during this process.