在纤维蛋白凝块上诱导脐血间充质干细胞向膀胱尿路上皮细胞的分化

Induced differentiation of umbilical cord blood mesenchymal stem cells into urothelial cells on fibrin clots

背景:在纤维蛋白凝块上诱导脐血间充质干细胞分化为膀胱尿路上皮细胞,尚无明确方案。目的:探讨脐血来源间充质干细胞在纤维蛋白凝块上分化为膀胱尿路上皮细胞的可能性。方法:实验组将脐血间充质干细胞接种于人工制备的脐血来源的纤维蛋白凝块上与胎儿膀胱尿路上皮细胞共培养,对照组将脐血间充质干细胞接种于人工制备的脐血来源的纤维蛋白凝胶上仅用DMEM/F1培养基培养。结果与结论:实验组的脐血间充质干细胞逐渐伸展呈多角形,细胞扁平变大,透射电子显微镜下具有膀胱尿路上皮细胞的超微结构,抗广谱角蛋白(AE1/AE3)免疫组织化学染色阳性。对照组的脐血间充质干细胞呈长梭形,抗广谱角蛋白(CKAE1/AE3)免疫组织化学染色阴性。提示在纤维蛋白凝块上诱导脐血间充质干细胞有分化为膀胱尿路上皮细胞的能力。

BACKGROUND： Whether umbilical cord blood mesenchymal stem cells （UCB-MSCs） can be induced to differentiate into urothelial cells on fibrin clots remains poorly understood. OBJECTIVE： To investigate the possibility of UCB-MSCs differentiating into urothelial cells on fibrin clots. METHODS： In the experimental group, the UCB-MSCs on umbilical cord blood fibrin clot were co-cultured with fetal urothelial cells. In the control group, the UCB-MSCs on umbilical cord blood fibrin clot were cultured in culture medium of DMEM/F12. RESULTS AND CONCLUSION： In the experimental group, the UCB-MSCs gradually became larger and were polygonal. The cells turned over their ultrastructure to urothelial cells under transmission electron microscope and were positive for immunhistochemistry staining of anti-cytokeratins （AE1/AE3）. In the control group, the UCB-MSCs morphology presented spindle-like arrangement and were negative for immunohistochemistry staining of anti-cytokeratins （AE1/AE3）. The UCB-MSCs on fibrin clot have the potential to differentiate into urothelial cells.