脂肪组织来源干细胞可促进脂肪移植物的存活率

Adipose tissue derived stem cells increase the survival rate of fat grafts

背景:在体外定向诱导条件下,脂肪组织来源干细胞可分泌细胞因子促进血管形成。目的:将脂肪组织来源干细胞和游离脂肪组织混合移植,评价脂肪组织来源干细胞对脂肪移植物存活率的影响。方法:采用不同感染复数MOI值Ad-EGFP基因转染人脂肪组织来源干细胞,确定理想的MOI值对细胞进行标记后,与人脂肪组织混合移植于裸鼠背部(实验组),以移植单纯脂肪组织为对照组。结果与结论:①脂肪组织来源干细胞转染24h可见绿色荧光,5d荧光表达强度最强,传代后仍有强荧光表达。MOI值为50时转染率95.3%较为理想。②移植后6个月实验组脂肪组织来源干细胞散在分布于部分脂肪细胞及小叶间隔中,在体内能够部分转化为血管内皮细胞,移植脂肪湿质量与存活率高于对照组(P=0.001),移植脂肪纤维化及坏死程度低于对照组(P=0.001)。说明脂肪组织来源干细胞移植在体内可促进游离移植脂肪组织的再血管化,提高移植脂肪组织存活率。

BACKGROUND： The adipose tissue derived stem cells （ADSCs） can secrete cell factors to promote neovascularization following directional induction in vitro. OBJECTIVE： To estimate the effects of ADSCs on the survival rate of fat grafts following the mixed transplantation of ADSCs and free fat tissues. METHODS： ADSCs were transfected by recombinant adenovirus encoding green fluorescent protein （Ad-EGFP） gene with different multiplicity of infection （MOI） values. Ad-EGFP-transfected cells at the ideal MOI were divided into experimental group and control group. Mixed transplantation of ADSCs and fat tissues into the back of nude mice was performed in the experimental group, and the simple fat tissues transplantation was taken in the control group. RESULTS AND CONCLUSION： Green fluorescence could be seen in ADSCs at 24 hours after transfection, the expression intensity at 5 days was the strongest, and the EGFP was still expressed after passage. The ideal transfection rate was 95.3% at a MOI value of 50. The ADSCs in the experimental group scattered in the fat cells and interlobular septum. Some of the ADSCs could be differentiated into vascular endothelial cells. The wet mass and survival rate of fat grafts in the experimental group were improved compared with the control group （P = 0.001）, but the fat graft fibrogenesis and damaged condition were decreased （P = 0.001）. The transplantation of ADSCs can increase the survival rate of fat grafts through promoting the angiopoiesis of free fat tissues.