胰岛素样生长因子1与神经干细胞源性神经元轴突的生长发育

Insulin-like growth factor 1 promotes axon growth in neural stem cell-derived neurons

背景：研究表明胰岛素样生长因子1具有神经保护作用并能增加神经干细胞向神经元及少突胶质细胞分化的比例。目的：观察胰岛素样生长因子1对神经干细胞源性神经元轴突生长发育的影响。方法：分离培养新生Wistar大鼠海马神经干细胞，传3~5代后接种于24孔培养板。其中12孔加入10 μL胰岛素样生长因子1（500 mg/L）作为实验组，余为对照组。结果与结论：培养1，2，3，4 d时，实验组细胞死亡数较对照组明显减少（P 〈 0.05），神经元轴突长度较对照组明显延长 （P 〈 0.05），但两组轴突的分叉数目差异无显著性意义（P 〉 0.05）。结果证实胰岛素样生长因子1可以增加神经干细胞向神经元的分化比例并促进神经干细胞源性神经元轴突长度的延伸，但不能增加轴突的分叉数量。

BACKGROUND： Previous studies have shown that insulin-like growth factor 1 has a neuroprotective effect and can promote the differentiation of neural stem cells into neurons and oligodendrocytes. OBJECTIVE： To investigate the effects of insulin-like growth factor 1 on axon growth in the neural stem cell-derived neurons. METHODS： Hippocampal neural stem cells of newborn wistar rats were cultured and passaged. Passage 3-5 neural stem cells were incubated in a 24-well plate. 10 μL insulin-like growth factors （500 mg/L） were added into 12 wells （experimental group）. The remaining wells served as control group. RESULTS AND CONCLUSION： At 1, 2, 3, 4 days of culture, dead cells in the experimental group were significantly fewer than those in the control group （P 〈 0.05）. The length of neuronal axon was significantly longer in the experimental group than in the control group （P 〈 0.05）, but there was no significant difference in bifurcation numbers between these two groups （P 〉 0.05）. These findings suggest that insulin-like growth factor 1 can increase the differentiation of neural stem cells into neurons and promote the extension of neural stem cells-derived neuronal axon, but it cannot increase the number of axon bifurcations.