肿瘤侵犯相关蛋白自分泌运动因子在瘢痕疙瘩中的表达

Expression of atuocrine motility factor in keloid

背景:肿瘤侵犯相关蛋白自分泌运动因子在肿瘤细胞中表达量增高,并可增强肿瘤细胞的生长、侵犯和转移能力,而瘢痕疙瘩具有恶性肿瘤生物学特性。目的:了解肿瘤侵犯相关蛋白自分泌运动因子在人体瘢痕疙瘩、增生性瘢痕、生理性瘢痕组织中的表达情况。方法:生理性瘢痕、增生性瘢痕、瘢痕疙瘩患者各20例,切取3种瘢痕组织;用组织免疫荧光染色法观测3种瘢痕组织中自分泌运动因子的分布及相对表达量;用蛋白质免疫印迹法和荧光定量PCR检测3种瘢痕组织中的自分泌运动因子蛋白水平和基因水平的表达。结果与结论:在组织水平、蛋白水平、基因水平上,生理性瘢痕和增生性瘢痕组织中自分泌运动因子的表达较低,且二者分布及相对表达量差异无显著性意义,而瘢痕疙瘩组织中,自分泌运动因子在3个水平上的表达强度明显增高,与前二者比较差异有显著性意义(P<0.01)。结果提示自分泌运动因子与瘢痕疙瘩的肿瘤生物学特征有着密切的关系。

BACKGROUND： Autocrine motility factor （AMF） exhibits an improved expression in tumor cells, and it can enhance cell growth, invasion and metastasis. But keloid has biological characteristics of malignant tumor. OBJECTIVE： To know about the expression of AMF in keloid, hypertrophic scar and normal scar tissues. METHODS： Each 20 cases of keloid, hypertrophic scar and normal scar were studied, and three kinds of scar tissues were selected. The distribution and relative expression of AMF in the three kinds of scar tissues were assessed by immunofluorescence. Protein and mRNA level of AMF in scar tissues were analyzed by western blot and real-time PCR methods. RESULTS AND CONCLUSION： In tissue, protein and mRNA levels, normal scar and hypertrophic scar tissues showed low expression of AMF. In distribution and relative expression, there was no significant difference between the normal scar and hypertrophic scar tissues. However, the keloid tissues showed significantly higher expression of AMF than normal scar and hypertrophic scar tissues in the three levels （P 〈 0.01）. These findings suggest that AMF is highly related to tumor biological characteristics of keloid.