A型肉毒毒素可抑制人增生性瘢痕成纤维细胞增殖和胶原蛋白的合成

Effect of botulinum toxin type A on the proliferation and collagen synthesis of human hypertrophic scar fibroblasts

背景:A型肉毒毒素伤口周围局部注射可以减少瘢痕的形成,而且对瘢痕的增生挛缩有抑制作用,可以促进瘢痕的萎缩变平。目的:观察A型肉毒毒素对人增生性瘢痕成纤维细胞的增殖和胶原蛋白合成的影响。方法:体外分离、培养人增生性瘢痕成纤维细胞,取对数生长期的细胞接种培养,使用稀释浓度为0.1U/LA型肉毒素DMEM细胞培养基对细胞的生长过程进行干扰,对照组以含胎牛血清的DMEM培养基培养。结果与结论:细胞接种第1～15天,对照组可见梭形的增生性瘢痕成纤维细胞分裂增殖,局部融合成细胞单层,细胞生长旺盛,细胞排列呈现高度一致性。A型肉毒毒素组细胞增殖速度慢,细胞数量少,细胞排列方向散乱,A型肉毒毒素组细胞数为对照组细胞数的79.3%,A型肉毒毒素实验组胶原蛋白合成量为正常对照组的48.4%,差异有显著性意义(P<0.05)。提示A型肉毒毒素可以抑制人增生性瘢痕成纤维细胞的增殖以及胶原蛋白的合成。

BACKGROUND： Local injection of botulinum toxin type A （BTA） around the wound can reduce the scar formation. The scar hyperplasia and contracture can also be inhibited; thereby the scar can become atrophic and flat. OBJECTIVE： To investigate the effect of BTA on the proliferation and collagen synthesis at human hypertrophic scar fibroblasts. METHODS： Human hypertrophic scar fibroblasts were isolated and cultured in vitro. The cells at logarithmic growth phase were cultured. Dulbecco＇s modified Eagle medium （DEME） containing BTA （diluted concentration of 0.1 U/L） was used to intervene the cell growth. Control group used DEME containing fetal bovine serum. RESULTS AND CONCLUSION： At 1 to 15 days after cells seeding, the hypertrophic scar fibroblasts showed the spindle-like shape with uniform composition and strong proliferation, the fibroblasts proliferated into monolayers, and the hypertrophic cells showed a high degree of consistency in the control group. The cells in the BTA group grew slowly and the arrangement of cells was scattered. The amount of cells in the BTA group was 79.3% of that in the control group. The collagen synthetic rate of BTA group was 48.4% of that in the control group. It is indicated that BTA can inhibit the proliferation and collagen synthesis of human hypertrophic scar fibroblasts.