糖尿病模型大鼠烫伤创面皮肤组织P物质和Bcl-2表达与龙血竭的干预

Effects of dragon's blood on the expression of substance P and Bcl-2 in wound tissue of diabetic scalded rats

背景:研究证明龙血竭有促进创伤愈合、抗血小板聚集、抗炎镇痛、抗菌抗氧化等生物活性。目的:验证龙血竭在糖尿病大鼠烫伤皮肤组织愈合过程中对P物质和Bcl-2表达的影响。方法:Wistar大鼠112只,随机分为4组。龙血竭组、磺胺嘧啶银(SD-Ag)组、糖尿病对照组和正常对照组。其中前3组为糖尿病深Ⅱ度烫伤模型,正常对照组为正常深Ⅱ度烫伤模型。结果与结论:烫伤后第7天开始创面愈合率龙血竭组、正常对照组高于SD-Ag组和糖尿病对照组(P<0.05),第21天龙血竭组创面愈合率明显高于SD-Ag组和糖尿病对照组(P<0.05),正常对照组最高。烫伤后第15天正常对照组P物质阳性表达水平最高,峰值显著高于其他3组,以后逐步减弱。龙血竭组、SD-Ag组和糖尿病对照组于烫伤后第21天阳性表达最强,且龙血竭组表达明显高于SD-Ag组和糖尿病对照组(P<0.05)。烫伤后第3天开始正常对照组Bcl-2阳性表达水平明显高于其他3组;于第15天各组阳性表达水平皆达到峰值,龙血竭组最高,明显高于SD-Ag组和糖尿病对照组(P<0.05),与正常对照组比较差异无显著性意义(P>0.05)。说明龙血竭能通过参与调控P物质和Bcl-2的表达,有效地促进糖尿病烫伤创面的愈合。

BACKGROUND： Previous studies have demonstrated that dragon’s blood can promote the wound healing as well as have characteristics of anti-platelet aggregation, anti-inflammatory analgesic, anti-bacterial and antioxidant and other biological activity. OBJECTIVE： To identify the effects of dragon’s blood on the expression of substance P and Bcl-2 in wound tissue of diabetic scalded rats. METHODS： A total of 112 Wistar rats were randomly divided into four groups： dragon’s blood group, silver sulfadiazine （SD-Ag） group, diabetic control group and normal control group. Diabetic deep Ⅱ degree burn models were established in dragon’s blood group, SD-Ag group and diabetic control group, and the normal deep Ⅱ degree burn models were established in normal control group. RESULTS AND CONCLUSION： On the 7th day post scald, the wound healing rates of dragon’s blood group and normal control group were higher than those of SD-Ag group and diabetic control group （P 〈 0.05）. After 21 days, the wound healing rate of dragon’s blood group was significantly higher than that of SD-Ag group and diabetic control group （P 〈 0.05） and highest in normal control group. After 15 days, the positive expression level of substance P reached a peak in normal control group and was higher than other three groups, and then decreased gradually. The expression was most positive in dragon’s blood group, SD-Ag group and diabetic control group at the 21th day post scald, and which was significantly higher in dragon’s blood group （P 〈 0.05）. On the 3rd day after scald, the expression level of Bcl-2 in the normal control group was significantly higher than that in other groups. From the 15th day, the expression of Bcl-2 was gradually increased in each group and reached to peak, and highest in dragon’s blood group （P 〈 0.05）, but there was no significant difference between dragon’s blood group and normal control group （P 〉 0.05）. The results demonstrate that dragon＇s blood can promote the healing of diabetic burn wounds significantly by regulating the expression of substance P and Bcl-2.