自体红骨髓构建非细胞型组织工程化骨修复骨缺损

Repairing bone defects by non-cell based tissue engineered complex constructed by autologous red bone marrow

背景:细胞型组织工程化骨修复骨缺损的效果良好,但存在操作复杂,容易污染及花费时间长等不足,不利于临床应用。目的:观察自体红骨髓构建的非细胞型组织工程化骨修复大段骨缺损的成骨效果。方法:在27只家兔一侧桡骨制作2cm大段骨缺损模型后,随机分3组,分别植入自体红骨髓+重组人胰岛素样生长因子1/珊瑚羟基磷灰石非细胞型组织工程化骨、自体红骨髓/珊瑚羟基磷灰石复合人工骨材料、珊瑚羟基磷灰石/重组人胰岛素样生长因子1复合人工骨材料。结果与结论:植入后4,8,12周,从植入物内部血管化程度、骨小梁数量、成熟骨结构形成及植入物的降解分析比较,自体红骨髓+重组人胰岛素样生长因子1/珊瑚羟基磷灰石非细胞型组织工程化骨组成骨能力及材料降解明显优于其他两组(P<0.05)。表明自体红骨髓构建的非细胞型组织工程化骨具有较高的成骨活性,修复大段骨缺损有显著效果。

BACKGROUND： Cell based tissue engineered bone is successful in the repair of bone defects, but it is a complex operation, easy to be contaminated and takes a long time, and not conducive to clinical application. OBJECTIVE： To investigate the efficacy of repairing large-segment bone defects by non-cell based tissue engineered complex constructed by autologous red bone marrow. METHODS： Twenty-seven rabbits were used to prepare 2 cm unilateral radial large-segment bone defect models, and then randomly divided into group A, group B and group C. Group A was implanted with autologous red bone marrow to construct tissue engineered bone （autologous red bone marrow＋recombinant human insulin-like growth factor-l/coralline hydroxyapatite）; group B was implanted with autologous red bone marrow/coralline hydroxyapatite artificial bone material; group C was implanted with coralline hydroxyapatite/recombinant human insulin-like growth factor-1 artificial bone material. RESULTS AND CONCLUSION： Group A was superior to the other two group in terms of osteogenic potential and material degradation by comparison of degree of bone formation, bone trabecular number, the structure formation of mature bone and implant degradation at 4, 8, 12 weeks after implantation （P 〈 0.05）. Non-cell based tissue engineered bone constructed by autologous red bone marrow has better osteogenic activity, and effective to repair large-segment bone defects.