类金刚石涂层瓣膜与犬血管内皮细胞的相容性

Compatibility of diamond-like carbon coating valves and canines vascular endothelial cells

背景:脉冲激光法沉积的类金刚石薄膜与低温各向同性碳同为非晶体碳质材料,具有良好的物理特性和生物相容性,且制作简单,成为现阶段构建组织工程化瓣膜的可选材料。目的:观察原代培养犬静脉血管内皮细胞与脉冲激光法沉积类金刚石薄膜瓣膜的生物相容性。方法:分离培养杂种犬前肢静脉血管内皮细胞,与脉冲激光法沉积的类金刚石薄膜纳米涂层瓣膜共同培养,以单纯犬前肢静脉血管内皮细胞为对照。结果与结论:两组细胞一氧化氮分泌功能无差别。倒置显微镜观察类金刚石薄膜材料周边血管内皮细胞未见衰老和异常核分裂现象。扫描电镜见血管内皮细胞在类金刚石薄膜上黏附紧密,细胞伪足延伸,细胞间出现互相连接现象,有细胞相互融合成片,将材料表面基本覆盖。表明类金刚石薄膜纳米涂层瓣膜具有良好的细胞相容性。

BACKGROUND： Diamond-like carbon film of pulsed laser method deposition and the low temperature isotropic carbon are considered as amorphous carbon. They are presented with good physical characteristics and biocompatibility, simple operation, and have become the selectable material for constructing tissue engineering valves at present. OBJECTIVE： To observe the biocompatibility of primary canine vein endothelial cells and diamond-like carbon valves prepared by pulsed laser deposition method. METHODS： Venous vascular endothelial cells from the forelimb of hybrid canines were isolated, and co-cultured with diamond-like carbon nano-coating valves prepared by pulsed laser deposition, serving as the experimental group. Pure canines forelimb vein vascular endothelial cells were as control group. RESULTS AND CONCLUSION： Nitric oxide secretory function of the two groups has no difference. Vascular endothelial ceils around diamond-like carbon film material were observed with the inverted microscope, and there was no aging and abnormal mitotic phenomenon. Scanning electron microscopy observation showed that vascular endothelial cells adhered on diamond-like carbon film closely; besides, cells were extended and interconnected with each other. Some cells were integrated together and diamond-like carbon film was almost covered. These findings suggest that diamond-like carbon nano-coating film has well biocompatibility.