仙灵骨葆对尾悬吊拟失重大鼠骨量丢失的抑制

Inhibition of Xianlinggubao on bone loss in tail-suspended rats

背景:以淫羊藿苷为主要成分的中药制剂仙灵骨葆具有促进骨形成的作用。目的:观察仙灵骨葆对尾悬吊拟失重大鼠骨量丢失的抑制作用及机制。方法:将SD大鼠随机分为正常对照组、拟失重组、仙灵骨葆组,后两组大鼠采用尾悬吊法模拟失重,仙灵骨葆组悬吊同时灌胃给予仙灵骨葆250mg/(kgd),拟失重组悬吊同时灌胃给予双蒸水,4周后取右侧股骨行骨密度、三点弯曲生物力学及骨组织形态计量学检测。结果与结论:正常对照组骨密度、胫骨近端骨小梁相对体积、骨小梁厚度、骨小梁数量、生物力学最大载荷显著高于拟失重组、仙灵骨葆组(P<0.05)。仙灵骨葆组胫骨远端1/4骨密度、胫骨近端骨小梁相对体积显著高于拟失重组(P<0.05),生物力学最大载荷也高于拟失重组,但差异无显著性意义。说明仙灵骨葆灌胃干预可促进松质骨的骨形成,部分阻止拟失重大鼠骨量的丢失。

BACKGROUND： Chinese herbal preparation Xian/inggubao whose main ingredient is icariin can promote bone formation. OBJECTIVE： To investigate the effect and related mechanism of Xian/inggubao on bone loss in tail-suspended rats. METHODS： Sprague-Dawley rats were divided into 3 groups： control group （A）, simulated weightlessness group （B） and Xian/inggubao group （C）. Rats in the group B and group C underwent tail-suspension, and in the group C was administered Xian/inggubao by gavage with dosage of 250 mg/（kg,d） at the same time while in the group B was administered double distilled water as control. Bone mineral density （BMD）, biomechanical three-point bending and bone morphology of the right femurs were detected after 4 weeks removing the right femurs. RESULTS AND CONCLUSION： BMD, trabecular bone volume, bone trabecular thickness, trabecular number and biomechanics maximum load in the group A were markedly higher than those in groups B and C （P 〈 0.05）, BMD of tibia distal 1/4, trabecular bone volume of proximal tibia in group C were higher as compared to group B （P 〈 0.05）. Biomechanics maximum load in the group C was also higher than that of the group B, but there was no significant difference. Xian/inggubao inhibition by gavage can promote bone formation and partially prevent bone loss in tail-suspended rats.