小檗碱衍生物B-119抑制肿瘤及血管内皮细胞增殖的作用

Effect of Berberine Derivate B-119 on Inhibition of Tumor and Vascular Endothelial Cell Proliferation

目的探讨小檗碱衍生物B-119对肿瘤细胞的增殖抑制作用及对血管内皮细胞增殖、迁移和小管形成的影响。方法MTT法检测B-119对瘤细胞株MDA-MB-231、B16、K562、LLC、HT29、SMMC-7721、Colo205、HL60和血管内皮细胞ECV304的增殖抑制作用;划痕法测定B-119对血管内皮细胞ECV304迁移的影响;观察B-119对血管内皮细胞ECV304小管形成的影响。结果 B-119对8株肿瘤细胞具有不同程度的抑制作用,IC50值为0.76～26.83mg/L;B-119对ECV304的增殖抑制作用表现为时间、浓度依赖性,24,48,72h的IC50分别为59.22,12.08,3.31mg/L;药物干预组ECV304细胞迁移数减少,且随B-119浓度的增加,迁移抑制作用越强,B-119浓度为1.25,2.5,5mg/L干预24h后,细胞迁移数渐次减少,抑制率分别为49.29%,59.71%和71.94%(P<0.01);B-119对ECV304小管形成有明显的抑制作用,浓度为10,20,30mg/L时的小管形成抑制率分别为35.71%,57.14%和67.86%(P<0.01)。结论 B-119可抑制肿瘤细胞的增殖,并抑制血管内皮细胞的增殖、迁移和小管形成。

Objective To investigate the effect of berberine derivate B-119 against the proliferation of tumor cells and against the proliferation,migration and tube-like structure formation of vascular endothelial cells.Methods MTT assay was applied to screen the anti-proliferative effect of B-119 on 8 kinds of tumor cell and ECV304 cells in vitro.The impact of B-119 on the migration of ECV304 cells was measured by scarification test,and the impact of B-119 on tube-like structure formation of ECV304 cells was examined under microscope.Results B-119 had different degrees of inhibition to these 8 tumor cell lines,and the IC50 of B-119 to these tumor cells were 0.76~26.83 mg/L.B-119 could inhibit the proliferation of ECV304 cells in a concentration-dependent and time-dependent manner.After being treated with B-119 for 24 h,48 h and 72 h,MTT assay showed the IC50 of B-119 to ECV304 were 59.22,12.08 and 3.31 mg/L respectively.The migration of ECV304 was reduced,and the inhibition effect of B-119 on ECV304 cells was strengthened with the increase of concentrations.After being treated with B-119 at the concentrations of 1.25,2.5 and 5 mg/L for 24 h,the number of ECV304 cells migration was gradually decreased and the migration inhibition ratios were 49.29%,59.71% and 71.94% respectively（P0.01）.B-119 could inhibit the tube formation of ECV304 cells,After being treated with B-119 at the concentrations of 10,20 and 30 mg/L for 18 h,the tube formation inhibition ratios were 35.71%,57.14% and 67.86% respectively（P0.01）.Conclusions B-119 could inhibit the proliferation of 8 kinds of tumor cell and also inhibit the proliferation,migration and tube-like structure formation of ECV304 cells.