黄癸素对人口腔上皮样癌KB细胞株及化学诱导性仓鼠颊癌作用的研究

Antitumor Effect of Berberine α-hydroxyl-β-decanoylethyl Sulfonate on KB Cell and Hamster Oral Cancer Induced by Chemical

目的研究黄癸素对体外培养人口腔上皮样癌KB细胞及化学诱导仓鼠的口腔癌变抑制作用。方法 MTT法检测黄癸素对KB肿瘤细胞株的影响,计算24,48,72h的IC50。采用8周龄仓鼠,建立二甲基苯丙蒽(DMBA)诱发的颊囊癌模型,用黄癸素进行干预。结果黄癸素对KB细胞有抑制作用,24,48,72h的IC50分别为(12.74±0.13),(4.56±0.22),(2.88±0.11)mg/L;对KB细胞生长呈浓度、时间依赖性抑制作用。DMBA诱导的仓鼠在36,54,72mg/kg的黄癸素作用下,颊黏膜不典型增生与癌变的发生率有不同程度的降低,其中54,72mg/kg剂量组的病变总发生率分别为66.6%和53.3%,比模型组(86.6%)分别降低了20.0%和33.3%(P<0.01)。结论黄癸素能显著的抑制KB细胞生长,对化学物诱导的仓鼠口腔癌变也有抑制作用。

Objective To investigate the antitumor activity of HB on KB cell in vitro and the inhibitory effect of chemical-induced hamster oral cancer.Methods MTT assay was used to detect the antitumor activity of HB on KB cell in vitro and chemical induction hamster oral cancerous.Methods： MTT,and then IC50 was calculated at 24,48,72 hours after the assay.The DMBA-induced cheek pouch cancer model was established by hamster（age at 8 weeks） and HB was used to intervene.Results HB inhibited the proliferation of KB cell in vitro,and IC50 of 24,48,72 hours was（12.74±0.13）mg/L,（4.56±0.22）mg/L and（2.88±0.11）mg/L,respectively.The growth inhibition of HB on KB cell showed time and dose-dependent.The dysplasia incidence induced by HB 54,72 mg/kg on DMBA-induced hamster oral mucosa was 66.6% and 53.3%,respectively.Compared with the control group（86.6%）,it decreased 20% and 33.3%,respectively.Conclusion HB has significant inhibitory activity on KB cell and chemical-induced hamster oral cancer.