应用Percoll密度梯度分离法观察悬浮红细胞的保存效果

The observation of store effect of suspension red blood cell by using percoll density gradient fractionation

目的:应用Percoll密度梯度分离法观察悬浮红细胞的保存效果,为临床安全用血,提高临床输血疗效提供依据。方法:应用Percoll密度梯度分离法对枸橼酸盐-磷酸盐-葡萄糖-腺嘌呤(CPDA)保存液,对保存4、7、14、21 d的悬浮红细胞进行密度(胞龄)分离并对各种胞龄的细胞计数。结果:保存4、7和14 d的悬浮红细胞在Percoll法下可以分成4种胞龄的红细胞,红细胞数目中龄1(M1)>中龄2(M2)>青龄(Y)>老龄(O)。保存21 d的红细胞只能分成3层,没有青龄红细胞。结论:悬浮红细胞在保存14 d后其青龄的红细胞逐渐减少,剩下中、老龄的红细胞。

Aim：To supply a better and more safer blood component therapy by using Percoll density gradient fractionation to observe the store effect of suspension red blood cell.Methods： Fractionate and count red blood cell in CPDA preserving fluid stored in（4±2）℃ according to their density（cell age）（4 days,7 days,14 days and 21 days separately） by using Percoll density gradient fractionation.Results： The suspension red blood cell stored in 4 days,7 days and 14 days can be fractionated to 4 layers according to their density（cell age）： the amount of middle age 1（M1） RBC middle age 2（M2） young age（Y） old age（O）.The suspension red blood cell stored in 21 days could only be fractionated to 3 layers,and did not contain young age red blood cell any more.Conclusion：The young age red blood cell of suspension red blood cell stored after 14 days gradually reduced with only middle age and old age red blood cell left.