岛津蒸发光散射检测器使用探讨

Disscussion on using of Shimadzu Evaporative Light Scattering Detector

蒸发光散射检测器是用于高效液相色谱法(HPLC)的通用检测器,属于质量型检测器,通过蒸发流动相,将目的化合物微粒子化,再使用光电倍增管测定强光照射后这些微粒子产生的散射光的方法。理论上可以检测到挥发性低于流动相的任何物质,特别适合检测糖类、脂肪、表面活性剂等没有紫外吸收的物质。岛津的蒸发光散射检测系统由于实现了低温下工作,具有优良的稳定性和操作简便性。

Evaporative light scattering detector is a common detector for high performance liquid chromatography （ HPLC）, which belongs to the quality - type detector. Through the evaporation mobile phase, compounds will become particles, and then using scatter- ing light tube measure the bright light exposure. After that, the method of scattering light produced by those particles, it can detect any substance which evoporate lower than mobile phase. Particularly suitable for the detection of carbohydrate, fat, surface active agent ete, and those has no Ultraviolet rays absorbing material. Dao Jing＇s evaporative light scattering detecting system has good stability and simple operation because of the relization of working in low temperature.