赤潮异弯藻对孔石莼生长及其某些生理特性的影响

Effects of Heterosigma akashiwo(Hada) Hada on the growth and physiological characteristics of Ulva pertusa Kjellm

通过实验生态学和生物化学的方法,研究了赤潮异弯藻[Heterosigma akashiwo(Hada)Hada]对孔石莼(Ulva pertusa Kjellman)生长及其叶绿素a(Chl-a)含量、丙二醛(MDA)含量、总抗氧化能力(T-AOC)、过氧化氢酶(CAT)活力、超氧化物歧化酶(SOD)活力和谷胱甘肽过氧化酶(GPX)活力的影响。结果表明:孔石莼的克生作用会明显抑制赤潮异弯藻的生长,同时赤潮异弯藻也会抑制孔石莼的生长,且孔石莼生理生化特性对其有明显的响应。赤潮异弯藻会使孔石莼CAT活力和MDA含量呈现升高的趋势;GPX活力呈现下降的趋势;T-AOC和T-SOD活力呈现先下降后升高趋势;Chl-a含量并无明显变化。推测赤潮异弯藻克生作用诱导孔石莼产生活性氧自由基,是藻体受损伤的主要原因。

The effects of Heterosigma akashiwo（Hada）Hada on the growth,physiological characteristics including Chl-a content,MDA content,T-AOC,CAT activity,SOD activity and GPX activity of Ulva pertusa Kjellman were studied by experimental ecology and biochemical methods.The results demonstrated that U.pertusa could inhibit the growth of H.akashiwo,and H.akashiwo could inhibit the growth of U.pertusa simultaneously.The effects of U.pertusa on physiological characteristics were obvious.The MDA content and CAT activity showed increasing trend,but GPX activity showed decreasing trend while T-AOC and T-SOD showed decreasing trend from 3d to 6d and increasing trend from 9d to 12d under allelopathy from H.akashiwo.The change of Chl-a content was not obvious.Superfluous reactive oxygen species produced by allelopathy from H.akashiwo was main reasons for the damage of U.pertusa.