卡介菌多糖核酸对肾病大鼠血清IL-4和IL-12的影响

Effects of BCG-PSN to serum levels of IL-4 and IL-12 in rats of nephrotic syndrome

目的:通过肾病综合征大鼠血清IL-4、IL-12的动态变化,探讨微小病变型肾病综合征(MCNS)的免疫学发病机制,揭示卡介菌多糖核酸(BCG-PNS)防治肾病可能途径。方法:本研究将40只大鼠随机分为正常组、肾病组、治疗组和预防组,每组10只。阿霉素5mg/kg制MCNS模型,BCG-PNS进行防治。观察大鼠一般情况,1、2、4周测24小时尿蛋白,2、4周取血生化仪测定血清白蛋白和总胆固醇水平,ELISA方法检测血清IL-4、IL-12的水平。实验结束时观察大鼠肾脏形态学改变。结果:1与正常组比较,肾病组出现大量的蛋白尿,低白蛋白血症,高胆固醇血症和明显的水肿,血清IL-4升高,IL-12降低,且均呈持续性加重。阿霉素注射4周,治疗组和预防组除IL-12与正常组无显著差异外,其它均有显著性差异。2与肾病组比较,治疗组在阿霉素注射4周,各指标均有显著性改善。预防组在阿霉素注射后2周与同期肾病组比较各指标均存在显著性差异。结论:1MCNS存在TH1/TH2功能紊乱,IL-4、IL-12参与了MCNS的免疫机制。2BCG-PNS可能通过诱导TH1型反应,促进IL-12的分泌,抑制TH2型反应,使IL-4分泌减少,而调整TH1/TH2的平衡,为MCNS防治提供实验依据。

Objective：To observe developing changes of serum value IL-12,IL-4 in rats of minimal change nephrotic syndrome（MCNS）.Probe MCNS immunopathogenesis and reveal possible treatment pathway of BCG-PNS to NS.Methods：Fourty male 6w wistar rats were randomly divided into normal group,nephrotic group,treated group,prevented group.（10 rats in each group）.MCN models were induced by adriamycin at a dose of 5mg/kg and treated and prevented with BCG-PSN.Treated group were used with medicine a after one week＇s adriamycin injection.Each group＇s normal conditions were observed.24h urinary protein were collected 1,2,4 week end.Serum albumin and serum total cholesterone were measured at 2,4 week end.IL-4,IL-12 serum level were measured with ELISA and every group renal morph logic were observed after the experiment was completed.Resultss：①there were quantitative proteinuria,hyperlipidemia,hypoalbuminemia and evidence edema.Serum il-4 level was higher,but serum IL-12 level was lower in nephrotic group than those of normal.② At 4 weeks end injection with adriamycin,treatment group and prevention group compared with the normal group,in addition to IL-12 no significant differences,other differences were significant.In comparison with nephrotic group,each index significantly increased in BCG-PSN treated group at 4 week end,which had obvious differences in prevented group comparing to nephrotic group at the according time.Conclusion：①Th1/Th2 functional imbalance exists in patients with MCNS,IL-4 and IL-12 are involved in the immunologic mechanisms.②BCG-PSN regulate the balance of Th1/Th2 possibly via induction Th1 phenotype to increase IL-12 secretes,suppressing Th2 phenotype to decrease IL-4 secretes.At the same time through improving body＇s disease,for the clinical and subsidiary curer to pediatric MCNS with BCG-PSN from immunology function angulation.