胃癌中NF-кB与COX-2表达的研究

Study on expressions of NF-кB and COX-2 in gastric carcinoma

目的探讨NF-кB、COX-2的表达与胃癌的关系及临床意义。方法采用免疫组织化学方法(S-P法)对胃癌组织、正常胃黏膜中NF-кB p65及COX-2蛋白表达进行检测。结果 NF-кB p65蛋白和COX-2蛋白在胃癌组织中的阳性表达率分别为67%和60%,正常胃黏膜中的阳性表达率分别为21%和18%,2者比较均有显著性差异(P均<0.01)。在低分化组的阳性表达率分别为79%和72%,高/中分化组的阳性表达率分别为53%和47%,2者比较均有显著性差异(P均<0.05);在浸润深度为T3+T4组的阳性表达率分别为73%和66%,T1+T2组的阳性表达率分别为22%和22%,2者比较均有显著性差异(P<0.01);在临床Ⅲ～Ⅳ期组的阳性表达率分别为78%和74%,Ⅰ～Ⅱ期组的阳性表达率分别为48%和37%,2者比较均有显著性差异(P均<0.01)。不同性别、年龄、肿瘤部位及大体分型胃癌组织中NF-кB p65和COX-2蛋白的表达无显著性差异(P均>0.05)。胃癌组织中NF-кB p65和COX-2蛋白的表达呈明显正相关(rs=0.539,P<0.01)。结论胃癌组织中的NF-кB和COX-2表达增高,提示其与胃癌的发生、发展、侵袭、淋巴转移密切相关。

Objective It is to approach the relationships of expression of NF-кB,COX-2 with gastric carcinoma and it＇s clinical significance.Methods The S-P immunohistochemical technique was used to detect the expressions of protein NF-кB p65 and COX-2 in gastric carcinoma tissues and normal gastric mucosa.Results The positive expression rate of NF-кB p65 and COX-2 was 67% and 60% respectively in gastric carcinoma,and was 21% and 18% respectively in normal gastric mucosa,there were significant differences（P0.01）.It was 79% and 72% respectively in poorly differentiated and was 53% and 47% in moderately and well differentiated,there were significant differences（P0.05）.It was 73% and 66% respectively in T3 ＋ T4 and was 22% and 22% respectively in T1 ＋ T2,there were significant differences（P0.01）.It was 78% and 74% respectively in Ⅲ~Ⅳ grade and was 48% and 37% respectively in Ⅰ~Ⅱ grade,there were significant differences（P0.01）.There was no significant difference in expressions of protein NF-кB p65 and COX-2 in gastric carcinoma tissues between distinct genders,age,locus and typing（all P0.05）.The expression of NF-кB p65 was positively correlated to COX-2（rs=0.539,P0.01）.Conclusion The expressions of NF-кB and COX-2 are higher in gastric carcinoma tissues,so they are related to genesis,development,invasion and metastasis of gastric carcinoma.