PTEN、p16和CyclinD1在胃癌及癌前病变中表达的研究

Study on expressions of PTEN,p16 and CyclinD1 in gastric carcinoma and precancerous lesion

目的探讨PTEN、p16和CyclinD1表达与胃癌发生发展的关系。方法应用免疫组化S-P法检测正常胃黏膜、慢性萎缩性胃炎、胃黏膜不典型增生及胃癌组织中PTEN、p16和CyclinD1蛋白的表达情况。结果 PTEN表达从正常胃黏膜、慢性萎缩性胃炎、胃黏膜不典型增生至胃癌,阳性表达率逐渐降低,不典型增生组阳性表达率显著高于胃癌组(P<0.05);p16在慢性萎缩性胃炎组中阳性表达率显著高于不典型增生组(P<0.05);CyclinD1在不典型增生组中阳性表达率显著低于胃癌组(P<0.05)。在胃癌组织中PTEN表达和p16呈正相关(rs=0.585,P<0.01),和CyclinD1呈负相关(rs=-0.587,P<0.01),p16表达与CyclinD1呈负相关(rs=-0.522,P<0.01)。结论胃癌发生机制涉及PTEN、p16和Cy-clinD1等基因的异常,随着病变进展,PTEN、p16表达降低,CyclinD1表达升高,提示PTEN、p16和CyclinD1可能是胃癌早期分子事件。

Objective It is to approach the relationships of protein expression of PTEN,p16 and CyclinD1 with development of gastric carcinoma.Methods The expression of PTEN,p16 and CyclinD1 in normal gastric mucosa,chronic atrophic gastritis,atypical hyperplasia and gastric carcinoma were detected by S-P immunohistochemical method.Results The positive expression rate of PTEN protein showed lower trend from normal gastric mucosa to gastric carcinoma.The positive expression rate of PTEN protein in atypical hyperplasia was significantly higher than that in gastric carcinoma（P0.05）.The positive expression rate of p16 protein in chronic atrophic gastritis was significantly higher than that in atypical hyperplasia（P0.05）.The positive expression rate of CyclinD1 protein in atypical hyperpl was significantly lower than that in gastric carcinoma（P0.05）.There was a positive relationship between PTEN and p16（rs= 0.585,P0.01）,a negative relationship between PTEN and CyclinD1（rs=-0.587,P0.01）,and a negative relationship between p16 and CyclinD1（rs=-0.522,P0.01）.Conclusion Abnormality of PTEN,p16 and CyclinD1 may be involved in molecular mechanism of gastric carcinogenesis,the positive expression of PTEN and p16 shows lower trend and the positive expression of CyclinD1 shows higher trend with the pathology progress,these suggeste that low level of PTEN and p16 protein expression and high level of CyclinD1 protein expression may be an early molecular event in gastric carcinogenesis.