黄芪扶正汤对Lewis肺癌荷瘤小鼠血清IL-2、IFN-γ表达的影响

Effect of Huangqi Fuzheng decoction on expression of serum IL-2 and IFN-γ in Lewis lung cancer tumor-bearing mice

目的探讨黄芪扶正汤对Lewis肺癌小鼠治疗作用的具体机制。方法建立肺癌荷瘤小鼠动物模型,以MTT法测脾脏T淋巴细胞增殖反应,ELISA法测血清IL-2和IFN-γ含量,评价黄芪扶正汤的抗肿瘤和免疫调节的作用,并初步分析其作用机制。结果黄芪扶正汤可以提高肺癌小鼠的脾脏指数,而对其T淋巴细胞增殖的能力基本影响不大。联合化疗处理组小鼠体内的IL-2、IFN-γ上升。结论黄芪扶正汤可以通过上调血清IL-2、IFN-γ的含量来提高其免疫功能,从而发挥可能的抗肿瘤作用。

Objective It is to approach the mechanism of Huangqi Fuzheng decoction（HQFZ） on Lewis mice with lung cancer.Methods The model of tumor-bearing mice was made,and then T-lymphocyte proliferation was measured by MTT,and contents of IL-2 and IFN-γ were measured by ELISA.The effects of anti-cancer and immunoloregulation of HQFZ were evaluated and the mechanism of action was analyzed.Results The spleen index of tumor-bearing mice was increased by treating with HQFZ,but the influence of HQFZ on T lymphocyte proliferation was little.The levels of serum IL-2 and IFN-γ of mice in DDP plus HQFZ group were increased.Conclusion HQFZ can improve immune function by up-regulate the levels of serum IL-2 and IFN-γ,this may play a role in anti-tumor effect.