黄酮类药物与氯金酸的作用及其分析应用

Redox of Flavonoids with Chlorauric Acid and Its Analytical Application

将4种黄酮类药物染料木素、木犀草素、大豆素及柚皮素与氯金酸在不同酸度条件下反应形成金纳米颗粒.研究发现,生成的金纳米颗粒的等离子体共振吸收信号与4种黄酮类药物的浓度呈线性关系,从而建立了这4种典型黄酮类药物的测定方法.分析了黄酮类药物的分子结构并利用Materials-studio 4.0软件计算了各原子的电子云密度分布.结果表明,4种黄酮类药物与氯金酸作用的适宜酸度与黄酮类化合物结构中的酚羟基数和碱性氧的电子云密度分布有关.

Four flavonoids including genistein, luteolin, daidzein and naringenin can reduce HAuC14 to form gold nanoparticles in different acidity. There are good linear correlations between the signal of plasmon resonance absorption of gold nanoparticles and the concentration of four flavonoids. Thus, we developed a method to detect these four flavonoids respectively. By analyzing the molecular structure and calculating the electron cloud density distribution of the atoms using Materials-studio 4. 0, we found that suitable pH for the reaction of different flavonoids and HAuC14 was related to the number of phenolic hydroxyl group of flavonoid and electron cloud density distribution of alkaline oxygen atoms.