基于线粒体COⅢ基因分析厚壳贻贝(Mytilus coruscus)养殖群体遗传多样性

THE GENETIC DIVERSITY OF CULTURED MYTILUS CORUSCUS POPULATIONS BASED ON SEQUENCE ANALYSIS OF MITOCHONDRIAL COⅢ GENE

基于线粒体DNA的COⅢ基因序列对我国沿海重要经济贝类厚壳贻贝3个养殖群体(温州、宁德、福州)的遗传多样性和遗传结构进行了分析。由PCR扩增获得23个体的COⅢ基因787bp的部分序列,其多态性遗传参数统计显示,23个个体共检出21个单倍型,总群体单倍型多样性指数(Hd)为0.978,核苷酸多样性指数(Pi)为0.01045,平均核苷酸差异数(K)为8.534,三个群体均显示出较丰富的遗传多样性。遗传分化系数(Fst)表明,福州群体与宁德群体和温州群体间有一定程度的遗传分化,而宁德群体和温州群体间无遗传分化。

The genetic diversity and genetic structure of three cultured populations （Wenzhou of Zhejiang province, Ningde of Fujian province and Fuzhou of Fujian province） of Mytilus coruscus were determined using the mitochondrial gene sequencing technology （COⅢ）. The result revealed 21 haplotypes for total 23 individuals obtained using PCR method. The haplotype diversities （Hd）, nucleotide diversities （Pi） and the average nucleotide differences （K） were 0.978, 0.01045 and 8.534, respectively, which showed high genetic diversity among populations. The fixation indices （Fst） of three popu-lations showed that there was no genetic differentiation between Wenzhou and Ningde populations and some genetic differentiation between Fuzhou population and the other two populations.