静水压力诱导牙鲆(Paralichthys olivaceus)四倍体的条件优化

PRODUCTION OF VIABLE TETRAPLOID OLIVE FLOUNDER(PARALICHTHYS OLIVACEUS) BY HYDROSTATIC PRESSURE SHOCK

采用静水压力抑制受精卵第一次卵裂的方法,进行了诱导牙鲆四倍体的研究。结果表明,将受精卵保持在(15.5±0.5)℃,从受精后70min开始用55MPa的压力处理6min后,正常仔鱼孵化率最高达到15.6%,此时的四倍体诱导率也最高达到63.3%。利用获得的最佳诱导条件处理获得了数千尾体长8—15cm的幼鱼,流式细胞仪检测和红血球长径测量结果显示,处理组幼鱼中四倍体约占13.3%,说明四倍体培育成功。该成果为开展牙鲆多倍体育种奠定了基础。

Viable tetraploid olive flounder （Paralichthys olivaceus） were hatched under hydrostatic pressure shock. The result showed that optimal hatching rate for tetraploid individuals was achieved by 6 min of pressure shock （55Mpa） at （15.5±0.5）℃, 70min after fertilization. Under these conditions, the hatching rate of the treated eggs was 15.6%. Chromo- some observation and counting of maximum number of nucleolus showed that the tetraploid rate was 63.3%. Several thousands of young fish with body length of 8--15cm were obtained using this method. Both flow-cytometry and erythro- cyte diameter proved that 13.3% of the young fish were tetraploids. The successful production of tetraploid individuals provided possibilities for future polyploid breeding using this fish species.