红曲中桔霉素的Ames试验

Ames Test of Citrinin in Monascus

采用鼠伤寒沙门氏菌试验(Ames试验),通过观察0.2、1.0、5.0、25.0μg/皿桔霉素处理对鼠伤寒沙门氏菌组氨酸缺陷型菌株TA97、TA98、TA100、TA102回复突变的影响,检测红曲中桔霉素的致突变性。结果表明,桔霉素受试物组回变菌落数均未超过自发回变菌落数的2倍,亦未呈现剂量反应关系,试验结果为阴性,红曲中桔霉素未表现出致突变性。

Ames test was applied to preliminarily study the mutagenicity of citrinin in monascus by observing the reverse mutation bacteria number of histidine-defective Salmonella typhimurium TA97,TA98,TA100 and TA102 treated by 0.2、 1.0、 5.0、 25.0 μg/vessel citrinin.Results showed that the reverse mutation bacteria number of treatment groups were less than two times of that in blank control;and no dose-response was presented.The test result was negative,indicating that citrinin had no mutagenicity under the experimental conditions.