摘要
为研究庐山楼梯草(Elatostema stewardii Merr.)总黄酮的提取工艺,以乙醇溶液为溶剂,在单因素试验基础上利用响应曲面法的中心组合设计,对庐山楼梯草总黄酮提取工艺参数进行优化分析。结果表明,提取庐山楼梯草总黄酮的最优工艺条件为乙醇体积分数55%、提取温度80℃、液料比37∶1(V/m,mL∶g)、提取时间2.4 h、提取2次,该条件下庐山楼梯草总黄酮提取率为5.14%。
Total flavonoids were extracted from Elatostema stewardii Merr.using alcohol as solvent.Single factor tests as well as response surface methodology based on a central composite design was applied to optimize the extraction process.The results showed that the optimized extraction conditions were volume fraction of alcohol solvent,55%;extraction temperature,80 ℃;solvent to material ratio,37∶1（V/m,mL∶g）;extraction time,2.4 h;and extract for twice.Under these extraction conditions,the extraction yield could be up to 5.14%.
出处
《湖北农业科学》
北大核心
2012年第8期1663-1666,1669,共5页
Hubei Agricultural Sciences
基金
九江学院2011年度大学生自主创新性实验项目
