中低压制备型色谱制备紫草萘醌成分的研究

Separation and Purification of Naphthaquinone Components From Rhizoma of Arnebia Euchroma(Royle) Johnst by Medium and Low Pressure Preparative Chromatography

目的建立新的色谱技术,分离纯化紫草萘醌成分。方法采用中低压制备色谱和Flash柱进行分离纯化,利用理化性质和核磁共振谱、红外、质谱等技术鉴定结构,用高效液相色谱技术分析单体的纯度。结果分离鉴定了6个化合物。分别为去氧紫草素,紫草素,异丁酰紫草素,乙酰紫草素,β-乙酰氧基异戊酰紫草素,β,β-二甲基丙烯酰紫草素,纯度分别是99.62%,99.02%,99.5%,99.31%,99.3%,99.2%。结论首次建立中低压色谱技术,快速、准确分离紫草药材的单体成分。

OBJECTIVE To establish a new chromatography technique to, separate and purify naphthaquinone components from rhizoma of Arnebia euchroma （Royle）. METHODS Medium and low pressure preparative chromatography and Flash column were used to separate and purify the constituents, and their structures were elucidated by physicochemieal properties, IR, MS, 1H-NMR and 13C-NMR. RESULTS Six monoeases were obtained, which were deoxyshikonin, shikonin, isobutylshikonin, aeetylshikonin, β-acetoxyisovalerylshikonin, and ββ-dimethylacrylshikonin. The purities of the 6 compounds analyzed by HPLC were 99. 62% ,99.02% , 99. 5% ,99.31% ,99. 3% and 99.2% , respectively. CONCLUSION A medium and low pressure preparative chromatography method has been established for the first time which can accurately and rapidly separate monocases from herbs.