补阳还五汤对大鼠全脑缺血再灌注的药效研究

Pharmacodynamics Study of Buyang Huanwu Decoction in Global Cerebral Ischemia-reperfusion Injury of Rats

【目的】比较补阳还五汤复方胶囊与原方汤剂保护和修复大鼠全脑缺血再灌注损伤神经细胞的作用,并从抗氧化角度探讨其作用机制。【方法】选用SD雄性大鼠,随机分为假手术组,模型组,补阳还五汤原方汤剂组(剂量为10 g.kg-1.d-1),复方胶囊高、中、低剂量组(剂量分别为7.56、3.78、1.89 g.kg-1.d-1);采用4血管闭塞法复制全脑缺血再灌注模型,比较补阳还五汤复方胶囊和原方汤剂对海马CA1区病理形态结构的修复程度,并对血清及脑组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及脑组织含水量进行测定。【结果】原方汤剂组及复方胶囊各剂量组对海马CA1区神经元细胞病理形态变化均有康复改善作用,且复方胶囊中剂量组作用最为显著;在血清和脑组织中,原方汤剂组及复方胶囊组均可使SOD活性显著升高,MDA含量显著下降(P<0.01),且以复方胶囊中剂量组作用最为明显;原方汤剂组及复方胶囊各剂量组均可显著降低脑组织含水量(P<0.01),其中复方胶囊高剂量组作用最明显。【结论】补阳还五汤原方汤剂及复方胶囊对大鼠全脑缺血再灌注损伤均有保护作用,但复方胶囊疗效更为确切。

Objective To compare the effect of the decoction and capsule of Buyang Huanwu Recipe（BHR） on protecting and repairing the neurons with global cerebral ischemia-reperfusion injury,and to explore the anti-oxidization mechanism.Methods Sprague-Dawley male rats were randomized into pseudo-operation group,model group,BHR decoction group（in the dosage of 10 g·kg-1·d-1）,and high-,moderate-and low-dosage BHR capsule groups（in the dosage of 7.56,3.78,and 1.89 g·kg-1·d-1）.Four-vessel occlusion method was used to establish the rat model of global cerebral ischemia-reperfusion injury.The effect of the decoction and capsule of BHR on the repair of pathological morphological structure of the hippocampal CA1 was observed.Meanwhile,serum and cerebral superoxide dismutase（SOD） activity and malondialdehyde content as well as the cerebral water content were measured.Results The decoction and capsule of BHR improved the pathological morphological structure of the hippocampal CA1,and the effect of the capsule was better.Serum and cerebral SOD activity was increased and MDA content was decreased in the decoction group and the capsule groups（P0.01 compared with the model group）,and the effect was better in moderate-dose BHR capsule group.Cerebral water content was decreased in the decoction and capsule of BHR groups（P0.01）,and the decrease was obvious in high-dosage BHR capsule group.Conclusion The decoction and capsule of BHR can protect the neurons from global cerebral ischemia-reperfusion injury,and the capsule has more reliable effect.