摘要
依据形态学特征结合rDNA-ITS分析,对采集到的野生真菌进行分类鉴定;采用RAPD分析生物学手段快速判断其分离菌株的真实性,并通过出菇试验验证判断结果。该野生真菌鉴定为赭鳞蘑菇(Agaricus subrufescens),RAPD分析和出菇试验结果表明分离菌株为该野生真菌的纯培养物。
Mushroom fruit bodies,collected from bamboo leaf piles near Bihu Town,Lishui City,Zhejiang Province,were identified as Agaricus subrufescens on the basis of sporophore and spore morphology,and ITS sequencing data.Pure cultures of fungal mycelium were obtained from fruit body tissue and confirmed as A.subrufescens on the basis of RAPD data and fruiting tests.
出处
《食用菌学报》
北大核心
2012年第1期27-35,共9页
Acta Edulis Fungi
基金
浙江省重大科技专项重点农业项目(编号:2009C12096)
浙江省食用菌产业创新团队项目(编号:2011R09029-13)的部分研究内容~~
关键词
野生真菌
赭鳞蘑菇
分离菌株
鉴定
Wild mushroom
Agaricus subrufescens
isolation of fungal mycelium
ITS sequencing
