1型糖尿病患儿血清维生素A浓度变化及其与Th1/Th2平衡相关性

Change of serum concentration of vitamin A and the balance of Th1/Th2 in children with type 1 diabetes mellitus

【目的】探讨1型糖尿病(type 1diabetes mellitus,T1DM)患儿血清维生素A浓度变化及其与辅助性T淋巴细胞Th1/Th2平衡相关性,了解维生素A在儿童TIDM中的作用。【方法】选取32例T1DM患儿为实验组,28例健康儿童为对照组,微量荧光法检测两组血清维生素A水平,酶联免疫吸附(ELISA)法检测培养上清液中干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)水平变化;【结果】1)T1DM组的血清维生素A水平明显低于对照组(P<0.01);2)T1DM组PBMC产生IFN-γ水平高于对照组(P<0.01),IFN-γ/IL-4比值明显高于对照组(P<0.01);3)二组血清维生素A浓度与PBMC产生IFN-γ水平皆呈负相关(P<0.05或<0.01),与IL-4水平均无相关性(P均>0.05),与IFN-γ/IL-4比值皆呈负相关(P<0.01或<0.01)。【结论】T1DM患儿存在维生素A缺乏,其与T1DM患儿Th1/Th2失衡关系密切。

【Objective】 To explore change of serum concentrtion of vitamin A,and its influence on the balance of Th1/Th2 in children with type 1 diabetes mellitus.【Methods】 Totally 32 children with T1DM were taken as T1DM group,and 28 healthy children as control group.The serum level of vitamin A was determined by the method of micro-flourescence,and the levels of IFN-γ and IL-4 in the supernatant were determined by enzyme-linked immunosorbent assay.【Results】 1）The level of vitamin A in T1DM group was lower than that in control group（P0.01）.2）IFN-γ level produced from PBMC in T1DM group was higher than that in normal control group（P0.05）,IFN-γ/IL-4 ratio was higher than that of normal control group（P0.01）.3）The levels of vitamin A in T1DM group and normal control group both had negative correlation with the levels of IFN-γ produced from PBMC（P0.05 or 0.01）,and negative correlation with the ratio of IFN-γ/IL-4（P0.05 or 0.01）,but had no correlation with the levels of IL-4（P0.05）.【Conclusions】 The deficiency of vitamin A is present in children with type 1 diabetes mellitus,which is closely correlated with the balance of Th1/Th2.