高羊茅种子盐溶蛋白的初步分析

Preliminary Analysis of Seed Salt Soluble Proteins in Tall Fescue(Festuca arundinacea)

利用改进的酸性聚丙烯酰胺凝胶电泳(Acid Polyacrylamide Gel Electrophoresis,A-PAGE)技术,对18个品种的高羊茅盐溶蛋白电泳图谱进行了统计分析。结果表明:供试材料具有较丰富的盐溶蛋白等位变异,共分离出17条相对迁移率不同的谱带,每份材料可电泳出7～12条带,材料间遗传相似系数(GS)变异范围在0.294～0.941,以相似系数0.635为标准,可将所有品种聚为3类。各品种的电泳图谱可以相互区别,表明种子盐溶蛋白酸性聚丙烯酰胺凝胶电泳图谱可作为高羊茅品种鉴定的蛋白质指纹图谱。

In this research, we analyzed the seed salt soluble protein of 18 Festuca arundinacea by improved Acide Polyacryamide Gel Electrophoreses （ A - PAGE）. The seed salt soluble protein electrophoregram was analyzed, the results shows that high level of salt soluble protein variations were presented in tall fescue. Totally separate 17 salt soluble protein bands with different migration rate, 7 -12 salt soluble protein were found in each material. Material genetic similarity （GS） vari- ation scope is between 0.294 ~ 0. 941, all of the specieses can be gathered with a standard of 0.635 GS. Each variety had its own unique band pattern distitin - guishable from the others. The electrophoregrams of A - PAGE of seed salt soluble pro- tein can be regarded as protein fingerprints for identification of tall fescue varieties.