北海市结核分枝杆菌耐药监测的培养结果分析

Analysis of culture results of mycobacterium tuberculosis drug resistance surveillance in Guangxi Beihai

目的比较初治组与复治组的培养阳性率(下称培阳率)和污染率以及二次培养对培阳率和污染率的影响。方法对60例初治和22例复治患者的2份涂阳痰标本接种于4支酸性罗氏培养基上进行培养。结果初治组与复治组的二次培养阳性率比较,差异有统计学意义(P<0.05)。初治组与复治组的污染率经二次培养后结果比较差异无统计学意义(χ2=1.15,P>0.05)。初治组、复治组和合计组初次培养和二次培养的培阳率比较,差异均无统计学意义(χ2=0.18,P>0.05;χ2=0.46,P>0.05;χ2=1.00,P>0.05)。初治组、复治组和合计组初次培养和二次培养的污染率比较差异均无统计学意义(χ2=0.29,P>0.05;χ2=0.36,P>0.05;χ2=0.64,P>0.05)。结论初治组的培阳率显著高于复治组,初治组的污染率与复治组差异无统计学意义。二次培养不能显著提高培阳率,也不能显著减少污染率。

Objective To Compared with the culture positive rate and the culture pollution rate of the group of first visit cases and the group of return visit cases as well as the influence of the second cultures to culture positive rate and the culture pollution rate.Methods Two smear positive sputum specimens in 60 patients with untreated and 22 patients with retreatment patients′ were inoculated in four acidic L G culture medium to culture.Results There was statistically difference of the second culture positive rate between the group of first visit cases and the return visit cases（χ2=5.46 P0.05）.There was no statistically difference of the culture pollution rate between the group of first visit cases and the return visit cases after the second cult（χ2=1.15,P0.05）.There was no statistically difference of he first and the second culture positive rate in the group of first visit cases,the group of return visit cases and the total group（χ2=0.18,P0.05;χ2=0.46,P0.05;χ2=1.00,P0.05）.There was no statistically difference of the first and the second culture pollution rate between the group of first visit cases,the return visit cases,and the total group（χ2=0.29,P0.05;χ2=0.36,P0.05;χ2=0.64,P0.05）.Conclusion Obviously,the culture positive rate of the group of first visit cases is much higher than that of the return visit cases.There are no significant differences between the culture pollution rate of the group of first visit cases and the one of the group of return visit cases.Second culture can neither raise the culture positive rate nor decline the culture pollution rate obviously.