水溶性黑柄炭角菌肽的体外抗氧化活性

Anti-oxidative activity of water-soluble peptides from Xylaria nigripes in vitro

目的研究水溶性黑柄炭角菌肽的体外抗氧化活性。方法通过测定水溶性黑柄炭角菌肽对邻苯三酚自氧化产生的超氧阴离子、Fenton反应产生的羟自由基、DPPH自由基的清除率,还原能力及抑制脂质体过氧化,全面考察水溶性黑柄炭角菌肽的体外抗氧化能力。结果水溶性黑柄炭角菌肽能明显降低邻苯三酚自氧化速率,对羟自由基有极强的清除能力,对DPPH自由基清除率随浓度增加而增加,其IC50分别为10、0.49 mg/mL和16.3μg/mL;进一步研究表明,黑柄炭角菌肽具有一定的还原力且与浓度呈正比例关系,同时也能抑制脂质体的过氧化反应。结论水溶性黑柄炭角菌肽在体外具有明显的抗氧化作用。

Objective To study anti-oxidative activity of water-soluble peptides from Xylaria nigripes（XNP） in vitro.Methods The anti-oxidative activity of water-soluble peptides from XNP were studied entirely by measuring scavenging rate of water-soluble peptides from XNP on super-oxide anion generated by pyrogallol autoxidation,hydroxyl free radical in Fenton reaction and DPPH free radical,and their reducing power and inhabiting peroxidation of liposomes ability.Results Water-soluble peptides from XNP could significantly reduce pyrogallol autoxidation rate,had highly scavenging capacity of hydroxyl free radical,and had dose dependent in scavenging DPPH free radical scavenging.Their IC50 were 10,0.49 mg/mL and 16.3 ug/mL respectively.Futher research showed that water-soluble peptides from XNP had certain reducing power increased as concentration increased,they also could inhibit the peroxidation of liposomes.Conclusion Water-soluble peptides from XNP was a good anti-oxidant in vitro test.