白背飞虱海藻糖合成酶基因的克隆及序列分析

Cloning and Sequence Analysis of Trehalose Phosphate Synthase Gene from Sogatella furcifera

为进一步深入研究白背飞虱的迁飞能力与能量代谢,利用同源克隆及RACE的方法,从白背飞虱Soga-tella furcifera中克隆获得海藻糖合成酶基因(TPS)的全长cDNA序列(Genbank登录号:JQ013797),该基因全长为3 278bp,包含353bp的3非编码区域(UTR)和501bp的5UTR,开放阅读框(ORF)为2 424bp,编码807个氨基酸。该基因预测的蛋白分子量为90.372kD,等电点为6.08,有3个预测的糖基化位点,无信号肽及跨膜结构。进化分析结果表明:白背飞虱的海藻糖合成酶与其它昆虫的海藻糖合成酶基因的同源性较高,与褐飞虱相比较同源性高达98%。

For the purpose of deep studying the migration ability and energy metabolism of Sogatella furcifera,the Trehalose Phosphate Synthasegene from Sogatella furcifera was cloned by homology cloning strategy and technique of Rapid-Amplification of cDNA Ends（RACE）.The full-length cDNA of S.furcifera TPS（SfTPS）was 3 278 bp（Genbank number：JQ 013797）and had an open reading frame（ORF）of 2 424 bp,a 3＇ UTR（untranslated region）of 353 bp and a 5＇UTR of 501 bp.The ORF of SfTPS encoded a polypeptide of 807 amino acids with a calculated molecular weight of 90.372 kD,and pl of 6.08.There were three N-glycosylation sites,but no signature peptide and putative transmembrane.A phylogenetic tree results using Blast showed that the amino acid sequence of SfTPS had the greatest similarity to other insects.Compared with Nilaparvata lugens,they were 98% identified.