秋水仙素对肇东苜蓿根尖细胞染色体的影响

Effect of Colchicine on Root Tip Chromosome in Zhaodong Alfalfa

为了探究秋水仙素对肇东苜蓿根尖细胞染色体的影响,利用不同浓度的秋水仙素(0.01%、0.05%、0.10%、0.15%和0.20%)以及处理时间(12、24、36和48h)分别对肇东苜蓿根尖细胞进行诱导,观察肇东苜蓿根尖细胞染色体、细胞中期分裂指数和细胞加倍指数的变化。结果表明:秋水仙素诱导了肇东苜蓿根尖细胞染色体的变异,细胞内染色体加倍,数目不等(32、64及128条);在时间与浓度互作方面,秋水仙素溶液浓度为0.15%时处理肇东苜蓿根尖12h,使细胞中期分裂指数达到最大值18.51%;利用浓度为0.10%的秋水仙素处理24h,细胞加倍指数达到最高值11.57%。

In order to understand the effect of colchicine on root tip chromosome of Zhaodong alfalfa,root tip cells of Zhaodong alfalfa were induced by different colchicines concentration（0.01%,0.05%,0.10%,0.15%,0.20%）and treatment duration（12,24,36,48 h）.The change ofchromosome,mitosis index and cell doubling index of Zhaodong alfalfa root tip cell were observed.The results showed that colchicine induced variation in Zhaodong alfalfa root tip cell chromosome doubling and the observed chromosome number was 32,64 and 128.The metaphase mitotic index was as high as 18.51% when root tip cell was treated for 12 hours in 0.15% colchicine.The cell doubling index reached 11.57% as tip cell was treated for 24 hours in 0.10% colchicine.