一种获取氧化修饰型低密度脂蛋白(Ox-LDL)的有效方法

An effective method of oxidation of low density lipoprotein

探讨了不同培养体系对血单核细胞源性巨噬细胞氧化修饰低密度脂蛋白能力的影响 ,旨在寻求一种获得Ox -LDL的有效方法。实验分为 4组 ,分别为HamF - 10、DMEM、DMEM +3μmol/LFe2 + 、DMEM +1μmol/L Cu2 + 。通过测定细胞存活率以及培养液上清中硫代巴比妥酸反应物 (TBARS) ,反映不同培养体系对巨噬细胞生长状态及氧化修饰LDL能力的影响。结果表明 :HamF - 10培养液对血单核细胞产生毒性 ,与DMEM培养液相比 ,细胞存活率显著降低 ;DMEM培养液中细胞生长良好 ,存活率高 ,但单纯DMEM培养液以及添加Fe2 + 的培养体系TBARS值显著低于添加Cu2 + 的DMEM培养体系 ,提示添加Cu2 + 的DMEM培养体系有利于巨噬细胞发挥氧化修饰LDL的能力。实验结果提示 :在研究巨噬细胞氧化修饰LDL的实验中 ,宜选用“DMEM +1μmol/LCu2 + ”的培养体系。

The effect of different media on oxidation of LDL by monocyte-derived macrophage system was investigated.The results showed that monocytes almost all turned into macrophages in DMEM medium whereas grew very badly and most of them died in Hams F-10 medium.Little or no modification of LDL took place when it was incubated with macrophages in DMEM,and adding 3 μmol/L Fe 2+ did not increase the modification of LDL by macrophages. Macrophages modify LDL well when they incubate in DMEM medium containing 1 μmol/L Cu 2+ , suggesting that Cu 2+ (1 μmol/L ) modifies LDL effectively when it is added to DMEM but not to Hams F-10. [WT5”HZ]