中药饮片对多重抗生素耐药细菌的抑菌作用

Inhibitory Effect of Chinese Herbal Medicine on Multiple Antibiotic-resistant Bacteria

目的:检测常见的28种清热解毒类中药饮片是否对抗生素抗性菌株与敏感菌株存在差别抑菌效果。方法:采用琼脂稀释法分别测定所选中药的水煎液对大肠杆菌和溶血葡萄球菌的敏感菌株和多重抗药突变菌株的抑菌情况。结果:大部分中药对抗生素抗性菌与敏感菌没有表现出明显的差别抑菌效果,表现差别抑菌效果的中药只有:连翘、厚朴、决明子、甘草。其中,抗生素抗性菌株往往比敏感菌株对中药更敏感。此外,不同中药的抑菌效果差别较大,对两种菌都有较好抑菌效果的是:黄连、黄芩、大黄、地榆、山茱萸、金银花、板蓝根、枸杞等。对两者抑菌效果都较差的中药包括:鱼腥草、桑白皮、山豆根、柴胡、蒲公英、防风、枇杷叶等。结论:只有少数中药对抗生素抗性菌与敏感菌存在差别抑菌效果,且抗性菌株往往比敏感菌株对中药更敏感。此结果提示中西医联合用药在治疗细菌感染性疾病分布可能更具优越性。

Objective： To detect whether common antipyretic and antidote Chinese Herbal Medicine have different inhibitory effects on antibiotic sensitive and resistant bacteria strains.Methods： The inhibitory effect of 28 Chinese Herbal Medicines,such as Yuxingcao（Houttuyniae herba）,Huanglian（Coptidis rhizome）,Huangqi（Scutellariae radix）,Dahuang（Rhei radix et rhizome）,Fangfeng（Saposhikoviae radix）,Jinyinhua（Lonicerea japonicae flos）,etc.,on antibiotic sensitive and resistant Escherichia coli and Staphylococcus haemolyticus strains were detected by agar dilution method.Results： The results showed that most of the 28 kinds of traditional Chinese medicine had no significant different inhibitory effect on antibiotic sensitive and resistant bacteria strains,only several had（4/28）,and they were Lianqiao（Forsythiae fructus）,Houpo（Magnoliae officinalis cortex）,Juemingzi（Cassiae semen）,Gancao（Glycyrrhizae radix et rhizoma）.Antibiotic resistant strains were usually more sensitive to the Chinese Herbal Medicine than the sensitive ones.Moreover,some Chinese Herbal Medicines had better inhibitory effects on both Escherichia coli and Staphylococcus haemolyticus strains,such as Huanglian（Coptidis rhizome）,Huangqin（Scutellariae radix）,Dahuang（Rhei radix et rhizome）,Jinyinhua（Lonicerea japonicae flos）,Diyu（Sanguisorbae radix）,Shanzhuyu（Corni fructus）,Banlangen（Isatidis radix）,etc.;Others had less inhibitory effects,such as Yuxingcao（Houttuyniae herba）,Sangbaipi（Mori cortex）,Shandougen（Sophorae tonkinensis radix et rhizome）,Fangfeng（Saposhnikoviae radix）,Pugongying（Taraxaci herba）,Pipaye（Eriobotryae folium）,etc..Conclusion： Only a small number of these 28 traditional Chinese medicines show different inhibitory effects on antibiotic sensitive and resistant bacteria strains,and the resistant strains are often more sensitive than the sensitive ones.The results suggest that the combination of traditional Chinese medicine and western medicine may have advantages in the treatment of bacterial infectious diseases.