睫状神经营养因子对严重烫伤大鼠纹状体及海马NOS阳性神经元的影响

EFFECTS OF CNTF ON NOS-POSITIVE NEURONS OF STRIATUM AND HIPPOCAMPUS IN BURNED RATS

目的：观察大鼠严重烫伤后纹状体和海马ＮＯＳ阳性神经元数目和阳性反应面积的变化及睫状神经营养因子（ＣＮＴＦ）对其的影响。方法：应用ＮＡＤＰＨ－ｄ酶组织化学的方法。结果：大鼠体表烫伤后３天，纹状体ＮＯＳ阳性神经元数目明显增加，染色呈强阳性，阳性反应面积增加。海马的ＮＯＳ阳性神经元数变化不明显，仅见阳性反应面积增加，睫状神经营养因子可降低纹状体的ＮＯＳ阳性神经元数目、阳性反应面积，ＮＯＳ阳性神经元着色较淡；海马的ＮＯＳ阳性反应面积也减少，睫状神经营养因子的作用与ＮＯＳ抑制剂Ｌ－ＮＡＭＥ的作用相似。结论；烫伤可引起大鼠脑内ＮＯＳ活性增加，睫状神经营养因子可能通过降低ＮＯＳ活性，从而减轻烫伤引起的神经元损伤。

To study the effects of CNTF on nitric oxide synthase NOS-positive neurons of striatum and hippocampus in burned rats. Method: Nicotinamide adenosine dinucleotide phosphate diaphorase (NADPH-d) enzyme histochemical staining is used. Result: The number of NOS positive neurons was significantly increased the staining was intense and the positive area was increased in striatum. The NOS positive area in hippocampus was also enlarged after burn, although the number of positive neurons didn't increase significantly. The effect could be attenuated after using CNTF, which was similar to L-NAME, a inhibitor to nitric oxide synthase. Conculsion: The above results indicated that CNTF could relieve neuron injury after burn through decreasing the activity of NOS.