竹类食用蕈菌——海绵胶煤炱菌子实体的纯培养

The Pure Culture of Fruiting Bodies of Scorias spongiosa,an Edible Mushroom of Bamboo

探索海绵胶煤炱菌子实体形成的培养条件,研究该菌的子实体发育规律,为规模化生产这种新型食用蕈菌提供技术方案。在浙江、四川、江苏等地共采集到100多份野生子实体标本,经组织分离得到纯培养菌株,在多种琼脂、液体、固体培养基上进行纯培养。在琼脂和固体培养基上,菌丝体直接分泌胶质物质形成胶质的小型子实体;在液体培养基上只形成菌丝和胶质菌丝球,不形成子实体。该菌的形态发育过程:分生孢子萌发→菌丝→胶质组织→子实体分枝→小型子实体→形成分生孢子器和分生孢子→释放孢子。海绵胶煤炱菌在纯培养条件下难以形成大型子实体。

The study explored the pure culture condition and development law of the fruiting bodies of Scorias spongiosa,in order to provide technical solutions for large-scale production of this new type of mushroom.More than 100 specimen of wild fruitbodies were collected from Zhejiang,Sichuan and Jiangsu province.Pure strains were obtained through tissus separation and pure culturing on agar,liquid and solid medium.Mycelium directly secreted gelatinous material to form colloidal fruitbodies in the agar and solid culture medium.In liquid medium,only mycelium and the mycelium sphere were formed,but not fruitbodies.The development process of this fungus was： conidia spore germination→ hyphal →colloid tissue→ branches of fruitbodies →small fruitbodies→ formation pycnidium and spores liquid group→ releasing spores.Under pure culture conditions it was difficult to form large fruiting bodies.