大量元素组成对孝顺竹苗组培快繁的影响

The Effects of Macronutrient Components on Tissue Culture and Rapid Propagation of Bambusa multiplex

为探索大量元素对竹子组培快繁的影响,以孝顺竹无菌苗为材料,采用L16(45)的正交试验设计,研究不同大量元素组成对孝顺竹组培苗扩繁效率的影响。结果表明,适宜孝顺竹增殖培养的大量元素组合为:1 650 mg.L-1NH4NO3+475 mg.L-1KNO3+220 mg.L-1CaCl2.2H2O+370 mg.L-1MgSO4.7H2O+85 mg.L-1KH2PO4;适宜孝顺竹生根培养的大量元素组合为:1 900 mg.L-1KNO3+220 mg.L-1CaCl2.2H2O+370 mg.L-1MgSO4.7H2O+85 mg.L-1KH2PO4。就不同成分而言,NH4NO3对孝顺竹继代增殖有促进作用,但抑制苗木的高生长。KH2PO4对苗木的增殖有抑制作用,KNO3对苗木的高生长有一定的抑制作用。

The L16（45） orthogonal experimental design was used to study the effects of macronutrient components on tissue culture and rapid propagation of Bambusa multiplex.The results showed that the combination of 1 650 mg·L-1 NH4NO3＋475 mg·L-1KNO3＋220 mg·L-1 CaCl2 2H2O ＋370 mg·L-1 MgSO4 7H2O ＋85 mg·L-1 KH2PO4 were suitiable for proliferation,while 1 900 mg·L-1KNO3＋220 mg·L-1CaCl2 2H2O＋370 mg·L-1 MgSO4 7H2O＋85 mg·L-1KH2PO4 were beneficial for rooting.NH4NO3 promoted proliferation and KH2PO4 inhibited proliferation of seedlings,NH4NO3 and KNO3 inhibited the growth of seedlings.